1
Frank DeRosa, Anusha Dias, Shrirang Karve, Michael Heartlein: Methods for purification of messenger RNA. Translate Bio, Fangli Chen, Proskauer Rose, December 26, 2017: US09850269

The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of (a) precipitating mRNA from an impure preparation; (b) subjecting the impure preparation comprising precipitated mRNA to a purification process involving membrane filtration such that ...


2
Michael Heartlein, Frank DeRosa, Anusha Dias, Shirang Karve: Methods for purification of messenger RNA. TRANSLATE BIO, Fangh Chen, Proskauer Rose, May 1, 2018: US09957499

The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of subjecting an impure preparation comprising in vitro synthesized mRNA to a denaturing condition, and purifying the mRNA from the impure preparation from step (a) by tangential flow fi ...


3
Frank DeRosa, Michael Heartlein, Anusha Dias: MRNA therapy for phenylketonuria. Shire Human Genetic Therapies, Proskauer Rose, Fangli Chen, December 20, 2016: US09522176

The present invention provides, among other things, methods of treating phenylketonuria (PKU), including administering to a subject in need of treatment a composition comprising an mRNA encoding phenylalanine hydroxylase (PAH) at an effective dose and an administration interval such that at least on ...


4
Michael Heartlein, Frank DeRosa, Anusha Dias: Quantitative assessment for cap efficiency of messenger RNA. TRANSLATE BIO, Fangli Chen, Proskauer Rose, May 15, 2018: US09970047

The present invention provides, among other things, methods of quantitating mRNA capping efficiency, particularly for mRNA synthesized in vitro. In some embodiments, the methods comprise chromatographic methods of quantifying capping efficiency and methylation status of the caps.