1
Samuel Davis, Eric Smith, Douglas MacDonald, Kara Louise Olson: Readily isolated bispecific antibodies with native immunoglobulin format. Regeneron Pharmaceuticals, Tor E Smeland, Yong Jin Choi, November 19, 2013: US08586713 (9 worldwide citation)

A bispecific antibody format providing ease of isolation is provided, comprising immunoglobulin heavy chain variable domains that are differentially modified in the CH3 domain, wherein the differential modifications are non-immunogenic or substantially non-immunogenic with respect to the CH3 modific ...


2
David Frendewey, Wojtek Auerbach, Ka Man Venus Lai, David M Valenzuela, George D Yancopoulos: Methods and compositions for the targeted modification of a genome. Regeneron Pharmaceuticals, Yong Jin Choi, Alston & Bird, January 5, 2016: US09228208 (6 worldwide citation)

Compositions and methods are provided for modifying a genomic locus of interest in a eukaryotic cell, a mammalian cell, a human cell or a non-human mammalian cell using a large targeting vector (LTVEC) comprising various endogenous or exogenous nucleic acid sequences as described herein. Further met ...


3
David Frendewey, David Jonathan Heslin, Ka Man Venus Lai, David M Valenzuela: miRNA-regulated differentiation-dependent self-deleting cassette. Regeneron Pharmaceuticals, Yong Jin Choi, Tor Smeland, Alston & Bird, August 4, 2015: US09096870 (4 worldwide citation)

Targeting constructs and methods of using them are provided for differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to a recombinase are provided, wherein the promoter drives transcription of t ...


4
Wojtek Auerbach, Thomas DeChiara, William Poueymirou, David Frendewey, David Valenzuela: Production of fertile XY female animals from XY ES cells. Regeneron Pharmaceuticals, Yong Jin Choi, Alston & Bird, July 26, 2016: US09398762 (3 worldwide citation)

Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will res ...


5
William Poueymirou, Thomas M DeChiara, Wojtek Auerbach, David Frendewey, David M Valenzuela: Conditioned culture media. Regeneron Pharmaceuticals, Tor E Smeland, Yong Jin Choi, Alston & Bird, August 26, 2014: US08816150 (3 worldwide citation)

Methods of generating modified embryos and mammals by introduction of donor cells into an early stage embryo are provided, such that the resulting embryo and animal generated therefrom has a significant contribution to all tissues from the donor cells and is capable of transmitting the donor cell DN ...


6
David Frendewey, Guochun Gong, Ka Man Venus Lai, David M Valenzuela: Promoter-regulated differentiation-dependent self-deleting cassette. Regeneron Pharmaceuticals, Yong Jin Choi, Tor Smeland, Alston & Bird, August 9, 2016: US09410163 (2 worldwide citation)

Targeting constructs and methods of using them are provided for differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to a recombinase are provided, wherein the promoter drives transcription of t ...


7
David Frendewey, David Jonathan Heslin, Ka Man Venus Lai, David M Valenzuela: MiRNA-regulated differentiation-dependent self-deleting cassette. Regeneron Pharmaceuticals, Yong Jin Choi, Tor Smeland, Alston & Bird, February 23, 2016: US09267152 (2 worldwide citation)

Targeting constructs and methods of using them are provided for differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to a recombinase are provided, wherein the promoter drives transcription of t ...


8
William Poueymirou, Thomas M DeChiara, Wojtek Auerbach, David Frendewey, David M Valenzuela: Methods and compositions for generating a mouse. REGENERON PHARMACEUTICALS, Tor E Smeland, Yong Jin Choi, Alston & Bird, August 16, 2016: US09414575 (2 worldwide citation)

Methods of generating modified embryos and mammals by introduction of donor cells into an early stage embryo are provided, such that the resulting embryo and animal generated therefrom has a significant contribution to all tissues from the donor cells and is capable of transmitting the donor cell DN ...


9
John McWhirter, Lynn MacDonald, Andrew J Murphy: Mice that produce antigen-binding proteins with pH-dependent binding characteristics. Regeneron Pharmaceuticals, Brownstein Hyatt Farber Schneck, Rita S Wu, Yong Jin Choi, April 5, 2016: US09301510 (1 worldwide citation)

Genetically modified non-human animals are provided that comprise an immunoglobulin heavy chain locus comprising an unrearranged human heavy chain variable region nucleotide sequence comprising an addition of at least one histidine codon or a substitution of at least one endogenous non-histidine cod ...


10
John McWhirter, Cagan Gurer, Karolina A Meagher, Lynn Macdonald, Andrew J Murphy: Non-human animals with modifed immunoglobulin heavy chain sequences. Regeneron Pharmaceuticals, Brownstein Hyatt Farber Schreck, Rita S Wu, Yong Jin Choi, December 8, 2015: US09204624 (1 worldwide citation)

Non-human animals, e.g., mammals, e.g., mice or rats, are provided comprising an immunoglobulin heavy chain locus that comprises a rearranged human immunoglobulin heavy chain variable region nucleotide sequence. The rearranged human immunoglobulin heavy chain variable region nucleotide sequence may ...