1
Kary B Mullis, Larry Johnson, Richard A Leath, Timothy J Wennberg, Louis M Mezei, Joseph T Widunas: System for automated performance of the polymerase chain reaction. The Perkin Elmer Corporation, William J Hone, Richard P Ferrara, August 12, 1997: US05656493 (315 worldwide citation)

There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing ...


2
Alexander B Chetverin, Helena V Chetverina: Method for amplification of nucleic acids in solid media. William J Hone, April 1, 1997: US05616478 (163 worldwide citation)

Amplification and/or expression of nucleic acids is carried out in a medium immobilized by using an organic and/or inorganic solid matrix penetrating the medium and having a porous, fibrous, reticulated, coiled, capillary, lamellar or folded texture and which includes the components of a cell-free e ...


3
Samuel Kayman, Abraham Pinter: Fusion glycoproteins. The Public Health Research Institute of the City of New York, William J Hone, July 1, 1997: US05643756 (26 worldwide citation)

Novel expression vectors are provided for expressing a fusion glycoprotein. The fusion glycoprotein contains the N-terminal globular domain of a retroviral env surface protein linked to a selected glycopeptide. Truncation glycoproteins as well as insertion glycoproteins are expressed using the vecto ...


4
Fred R Kramer, David Dubnau, Karl A Drlica, Abraham Pinter: Selection of ribozymes that efficiently cleave target RNA. The Public Health Research Institute of the City of New York, William J Hone, April 1, 1997: US05616459 (23 worldwide citation)

Mutant ribozymes are screened by culturing cells whose survival is dependant upon cleavage of RNA by a ribozyme, which cleavage causes the cells to survive in the presence of an agent which otherwise would kill the cells, and selecting cells which survive.


5
Ying Wu, Lyubov A Ryabova, Oleg V Kurnasov, Igor Y Morosov, Viktor I Ugarov, Elena V Volianik, Alexander B Chetverin, David Zhang, Fred R Kramer, Alexander S Spirin: Coupled replication-translation methods and kits for protein synthesis. William J Hone, September 17, 1996: US05556769 (5 worldwide citation)

Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Q.beta. replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupl ...


6
Antonius H W M Schuurs, Bauke K van Weemen: Process for the demonstration and determination of reaction components having specific binding affinity for each other. Akzona Incorporated, Robert H Falk, William J Hone, Charles A Wendel, August 3, 1982: USRE031006

The present invention relates to a process for the determination of a component of the reaction between a specific binding protein and the substance being specifically bound by such a protein comprising reacting the component to be determined with its binding partner in an insolubilized form, separa ...


7
Mark E Holman: Gel-coated materials with incereased flame retardancy. Frisby Technologies, William J Hone, Fish & Richardson PC, July 5, 2001: US20010006865-A1

The invention provides gel-coated materials that provide enhanced flame-, physical- and chemical-resistance to the foamed materials. The gel coatings can be created with a sol-gel process. Such treated materials can be used, for example, in the manufacture of articles of clothing that are to be used ...


8
Yan Xiong, Cullen M Sabin: Vacuum packaging aid. William J Hone, Fish & Richardson PC, November 1, 2001: US20010034999-A1

Vacuum packaging methods and materials are claimed. The materials are fusible and can form a part of a heat seal closure for non-rigid and semi-rigid packages. The methods are suitable for packages containing materials generally, and are well suited for those containing granular materials.


9
Cullen M Sabin, Martin W Sabin, Yan Xiong: Portable heating/cooling and dispensing devices. William J Hone, Fish & Richardson PC, November 8, 2001: US20010037872-A1

A self-contained device for heating/dispensing or cooling/dispensing material is described. The device includes at least one heat-or cold-generating element and at least one container for containing material to be heated or cooled. The device can further be equipped with gussets or similar features ...


10
Olga Marko, William K Boss: Treatment of tissue with undifferentiated mesenchymal cells. William J Hone, Fish & Richardson PC, May 27, 2004: US20040101959-A1

The present invention provides compositions and methods for correcting cosmetic, aesthetic, and degenerative defects in the skin, soft tissue, and bone of a subject. In particular, methods of the invention involve the injection or implantation of autologous UMC, fibroblasts, and/or keratinocytes int ...