Kary B Mullis, Larry Johnson, Richard A Leath, Timothy J Wennberg, Louis M Mezei, Joseph T Widunas: System for automated performance of the polymerase chain reaction. The Perkin Elmer Corporation, William J Hone, Richard P Ferrara, August 12, 1997: US05656493 (315 worldwide citation)

There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing ...

Alexander B Chetverin, Helena V Chetverina: Method for amplification of nucleic acids in solid media. William J Hone, April 1, 1997: US05616478 (163 worldwide citation)

Amplification and/or expression of nucleic acids is carried out in a medium immobilized by using an organic and/or inorganic solid matrix penetrating the medium and having a porous, fibrous, reticulated, coiled, capillary, lamellar or folded texture and which includes the components of a cell-free e ...

Samuel Kayman, Abraham Pinter: Fusion glycoproteins. The Public Health Research Institute of the City of New York, William J Hone, July 1, 1997: US05643756 (26 worldwide citation)

Novel expression vectors are provided for expressing a fusion glycoprotein. The fusion glycoprotein contains the N-terminal globular domain of a retroviral env surface protein linked to a selected glycopeptide. Truncation glycoproteins as well as insertion glycoproteins are expressed using the vecto ...

Fred R Kramer, David Dubnau, Karl A Drlica, Abraham Pinter: Selection of ribozymes that efficiently cleave target RNA. The Public Health Research Institute of the City of New York, William J Hone, April 1, 1997: US05616459 (23 worldwide citation)

Mutant ribozymes are screened by culturing cells whose survival is dependant upon cleavage of RNA by a ribozyme, which cleavage causes the cells to survive in the presence of an agent which otherwise would kill the cells, and selecting cells which survive.

Ying Wu, Lyubov A Ryabova, Oleg V Kurnasov, Igor Y Morosov, Viktor I Ugarov, Elena V Volianik, Alexander B Chetverin, David Zhang, Fred R Kramer, Alexander S Spirin: Coupled replication-translation methods and kits for protein synthesis. William J Hone, September 17, 1996: US05556769 (5 worldwide citation)

Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Q.beta. replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupl ...

Antonius H W M Schuurs, Bauke K van Weemen: Process for the demonstration and determination of reaction components having specific binding affinity for each other. Akzona Incorporated, Robert H Falk, William J Hone, Charles A Wendel, August 3, 1982: USRE031006

The present invention relates to a process for the determination of a component of the reaction between a specific binding protein and the substance being specifically bound by such a protein comprising reacting the component to be determined with its binding partner in an insolubilized form, separa ...

Mark E Holman: Gel-coated materials with incereased flame retardancy. Frisby Technologies, William J Hone, Fish & Richardson PC, July 5, 2001: US20010006865-A1

The invention provides gel-coated materials that provide enhanced flame-, physical- and chemical-resistance to the foamed materials. The gel coatings can be created with a sol-gel process. Such treated materials can be used, for example, in the manufacture of articles of clothing that are to be used ...

Yan Xiong, Cullen M Sabin: Vacuum packaging aid. William J Hone, Fish & Richardson PC, November 1, 2001: US20010034999-A1

Vacuum packaging methods and materials are claimed. The materials are fusible and can form a part of a heat seal closure for non-rigid and semi-rigid packages. The methods are suitable for packages containing materials generally, and are well suited for those containing granular materials.

Cullen M Sabin, Martin W Sabin, Yan Xiong: Portable heating/cooling and dispensing devices. William J Hone, Fish & Richardson PC, November 8, 2001: US20010037872-A1

A self-contained device for heating/dispensing or cooling/dispensing material is described. The device includes at least one heat-or cold-generating element and at least one container for containing material to be heated or cooled. The device can further be equipped with gussets or similar features ...

Olga Marko, William K Boss: Treatment of tissue with undifferentiated mesenchymal cells. William J Hone, Fish & Richardson PC, May 27, 2004: US20040101959-A1

The present invention provides compositions and methods for correcting cosmetic, aesthetic, and degenerative defects in the skin, soft tissue, and bone of a subject. In particular, methods of the invention involve the injection or implantation of autologous UMC, fibroblasts, and/or keratinocytes int ...