61
Frank A Dolbeare, Joe W Gray: Flow cytometric measurement of total DNA and incorporated halodeoxyuridine. The Regents of the University of California, Stephen C Macevicz, Shyamala T Rajender, Berthold J Weis, October 25, 1988: US04780406 (37 worldwide citation)

A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements ...


62
Joe W Gray, Daniel Pinkel: Method of preparing and applying single stranded DNA probes to double stranded target DNAs in situ. Regents of the University of California, Leona L Lauder, Henry P Sartorio, Stephen C Macevicz, July 2, 1991: US05028525 (35 worldwide citation)

A method is provided for producing single stranded non-self-complementary nucleic acid probes, and for treating target DNA for use therewith. Probe is constructed by treating DNA with a restriction enzyme and an exonuclease to form template/primers for a DNA polymerase. The digested strand is resynt ...


63
Edwin F Ullman, Sharat Singh: Methods for single nucleotide polymorphism detection. Aclara Biosciences, Stephen C Macevicz, October 14, 2003: US06632606 (35 worldwide citation)

Methods and compositions are provided for determining large numbers of single nucleotide polymorphisms in target DNA employing particles having (1) primers complementary to sequences in the target DNA where the next succeeding 3′-nucleotide is a potential single nucleotide polymorphism and coding co ...


64
Steven Michael Menchen, Mitchell A Winnik, Ben F Johnson: Viscous electrophoresis polymer medium and method. The Perkin Elmer Corporation, Peter J Dehlinger, Stephen C Macevicz, Vincent M Powers, June 2, 1998: US05759369 (33 worldwide citation)

An electrophoresis separation medium is disclosed comprising a matrix of a copolymer composed of a linear hydrophilic polymer segment having a selected substantially uniform segment length and hydrophobic polymer segments carried on each end. Also disclosed is an electrophoresis method employing the ...


65
Tracy J Matray, Vincent S Hernandez, Ahmed Chenna, Herbert Hooper, Sharat Singh: Methods for detecting a plurality of analytes by mass spectrometry. Aclara Biosciences, Stephen C Macevicz, November 18, 2003: US06649351 (32 worldwide citation)

The invention provides a method for detecting a target analyte, by: (a) contacting one or more target analytes with a set of first and second binding reagents under conditions sufficient for binding of a target analyte with the first and second binding reagents, each of the first binding reagents co ...


66
Sydney Brenner: Polynucleotide detection by isothermal amplification using cleavable oligonucleotides. Lynx Therapeutics, Stephen C Macevicz, Vincent M Powers, May 5, 1998: US05747255 (29 worldwide citation)

A method for detecting a target polynucleotide is provided which relies on the exponential amplification of oligonucleotide fragments. Separated populations of oligonucleotides are provided that contain complementary sequences to one another and that contain at least one scissile linkage which is cl ...


67
Sharat Singh, Po Ying Chan Hui: Multiplex analysis using membrane-bound sensitizers. Aclara Biosciences, Stephen C Macevicz, September 27, 2005: US06949347 (28 worldwide citation)

The present invention is directed to methods and compositions for determining the presence, absence, and/or amounts of one or more membrane-associated analytes in a sample. In accordance with the invention, binding compounds derivatized with releasable molecular tags specifically bind to selected me ...


68
Keith D Brown, Timothy K Mosmann, Gerard Zurawski, Sandra M Kurawski: T cell activation markers. Schering Corporation, Stephen C Macevicz, March 19, 1991: US05001230 (28 worldwide citation)

A polypeptide designated H400 and its encoding nucleic acid are provided as markers specific for activated human T cells. Activated t cells are detected immunochemically by monoclonal antibodies specific for H400 or its immunogenic peptides. Activated T cells are also detected by nucleic acid probes ...


69
Sharat Singh, Tracy Matray, Ahmed Chenna: Sets of generalized target-binding e-tag probes. Stephen C Macevicz, August 3, 2004: US06770439 (28 worldwide citation)

Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, c ...


70
Wojciech J Stec, Andrzej Grajkowski, Bogdan Uznanski: Solid phase oligonucleotide synthesis using phospholane intermediates. Polish Academy of Sciences, Stephen C Macevicz, April 30, 1996: US05512668 (28 worldwide citation)

Methods and compounds are provided for solid phase synthesis of oligonucleotides and related polymers by condensing protected monomer-O-[1,3,2-dichalcogen-substituted-phospholane] synthons in the presence of a catalytic base. Compounds of the invention include 2-N-substituted-1,3,2-dichalcogen-subst ...