11
Sydney Brenner: Method of sorting polynucleotides. Lynx Therapeutics, Stephen C Macevicz, January 26, 1999: US05863722 (165 worldwide citation)

The invention provides a method and materials for sorting polynucleotides with oligonucleotide tags. Oligonucleotide tags of the invention are capable of hybridizing to complementary oligomeric compounds consisting of subunits having enhanced binding strength and specificity as compared to natural o ...


12
John Bridgham, Kevin Corcoran, George Golda, Michael C Pallas, Sydney Brenner: System and apparatus for sequential processing of analytes. Lynx Therapeutics, Stephen C Macevicz, Vincent M Powers, LeeAnn Gorthey, November 25, 2003: US06654505 (159 worldwide citation)

An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside a flow chamber where steps of an ...


13
B John Bergot, Vergine Chakerian, Charles R Connell, J Scott Eadie, Steven Fung, N Davis Hershey, Linda G Lee, Steven M Menchen, Sam L Woo: Spectrally resolvable rhodamine dyes for nucleic acid sequence determination. Applied Biosystems, Stephen C Macevicz, Peter Dehlinger, Vince Powers, November 22, 1994: US05366860 (157 worldwide citation)

A spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. A different rhodamine dye from the group consisting of tetramethylrhodamine, rhodamine X, rhodamine 6G, and rhodamine 110 is attached to the base of each of the dideoxynucle ...


14
Sydney Brenner: Simultaneous sequencing of tagged polynucleotides. Lynx Therapeutics, Stephen C Macevicz, June 9, 1998: US05763175 (156 worldwide citation)

The invention provides a method for sequencing each polynucleotide of a population by using an oligonucleotide tag assigned to each such polynucleotide for transfering sequence information to a tag complement located on a spatially addressable array of such complements. That is, a unique tag is atta ...


15
Sydney Brenner: Minimally cross-hybridizing sets of oligonucleotide tags. Spectragen, Stephen C Macevicz, June 3, 1997: US05635400 (146 worldwide citation)

The invention provides a method of tracking, identifying, and/or sorting classes or subpopulations of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention each consist of a plurality of subunits 3 to 6 nucleotides in length selected from a minimally cross-hybridizing s ...


16
Sergei M Gryaznov, Ronald G Schultz, Jer Kang Chen: Oligodeoxyribonucleotide N3 P5 phosphoramidates. Lynx Therapeutics, Stephen C Macevicz, March 10, 1998: US05726297 (135 worldwide citation)

Modified oligodeoxyribonucleotide 3'--NHP (O) (O.sup.-) O-5' phosphoramidates were synthesized on a solid phase support. The phosphoramidate analogs were found to have significantly increased resistance toward phosphodiesterase digestion. Thermal dissociation experiments demonstrated that these comp ...


17
William A Andrus, Christie D McCollum, Gerald Zon: Automated system for polynucleotide synthesis and purification. Applied Biosystems, Joseph H Smith, Stephen C Macevicz, September 10, 1991: US05047524 (134 worldwide citation)

A method and system for polynucleotide synthesis and purification are provided which employ solid phase synthesis on a nonswellable porous polystyrene support by phosphoramidite or hydrogen phosphonate chemistries. The polystyrene support gives rise to fewer tritylated failure squences caused by cha ...


18
Sydney Brenner: Oligonucleotide tags for sorting and identification. Lynx Therapeutics, Stephen C Macevicz, January 9, 2001: US06172214 (133 worldwide citation)

The invention provides a method of tracking, identifying, and/or sorting classes or subpopulations of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention comprise oligonucleotides selected from a minimally cross-hybridizing set. Preferably, such oligonucleotides each ...


19
Wojciech J Stec, Bogdan Uznanski, B John Bergot, Bernard L Hirschbein, Karen L Fearon: Method of synethesizing sulfurized oligonucleotide analogs. Applied Biosystems, Stephen C Macevicz, September 29, 1992: US05151510 (128 worldwide citation)

A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiophosphorus compound, such as a thiophosphoric, dithiophosphoric, thiophosphinic, or dithiophosphinic acid disulfide or polysulfide, as a sulfurizing a ...


20
Sydney Brenner, Robert B DuBridge: DNA sequencing by stepwise ligation and cleavage. Lynx Therapeutics, Stephen C Macevicz, February 3, 1998: US05714330 (117 worldwide citation)

The invention provides a method of nucleic acid sequence analysis based on repeated cycles of ligation to and cleavage of probes at the terminus of a target polynucleotide. At each such cycle one or more terminal nucleotides are identified and one or more nucleotides are removed from the end of the ...