1
Brian Goodall
Alex M Aravanis, Brian L Goodall, Michael Mendez, Jason L Pyle, Jaime E Moreno: Methods and systems for biofuel production. Sapphire Energy, Sapphire Energy, November 25, 2010: US20100297749-A1

The present disclosure relates to methods and systems for biofuel production. Systems of integrated biorefineries (IBR) and methods of using IBRs for producing fuel compositions and other products are provided herein. The IBRs can use algae for generating biofeedstock to produce the fuel composition ...


2
Richard J Cranford, Alex M Aravanis, Stilianos G Roussis: Process for the recovery of oleaginous compounds from biomass. Sapphire Energy, Sapphire Energy, June 5, 2012: US08192628 (4 worldwide citation)

Disclosed herein are methods and processes for the recovery of oleaginous compounds from biomass and in particular biomass comprises photosynthetic microorganisms. Also disclosure are oleaginous compounds obtained using the disclosed methods.


3
Richard J Cranford, Alex M Aravanis, Stilianos G Roussis: Process for the recovery of oleaginous compounds from biomass. Sapphire Energy, Sapphire Energy, May 12, 2015: US09028696 (1 worldwide citation)

Disclosed herein are methods and processes for the recovery of oleaginous compounds from biomass and in particular biomass comprises photosynthetic microorganisms. Also disclosure are oleaginous compounds obtained using the disclosed methods.


4
Richard J Cranford, Alex M Aravanis, Stilianos G Roussis: Process for the recovery of oleaginous compounds and nutrients from biomass. Sapphire Energy, Sapphire Energy, December 9, 2014: US08906236

Disclosed herein are methods and processes for the recovery of nutrients from non-organic phases produced during recovery of oleaginous compounds from biomass. The nutrients recovered can then be utilized to grow additional biomass.


5
Brian L Goodall, Alex M Aravanis, Craig A Behnke, Richard J Cranford, Daniel J Sajkowski: Methods of preparing oil compositions for fuel refining. SAPPHIRE ENERGY, Sapphire Energy, September 29, 2015: US09145528

Disclosed herein are methods and systems for upgrading (for example, removing heteroatoms, metals, or metalloids) an oil composition derived or extracted from a biomass. The upgraded oil composition can be used to make a desired product, for example, a fuel product.


6
Stilianos G Roussis, Richard J Cranford: Compositions of matter comprising extracted algae oil. SAPPHIRE ENERGY, Sapphire Energy, December 1, 2015: US09200208

Crude algae oils produced by practical extraction techniques comprise a wide range of molecular species that can be characterized by advanced analytical techniques. The algae oils comprise a complex mixture of a large number of molecules having varying sizes and therefore varying boiling points, and ...


7
Michael Mendez, Bryan O Neill, Kari Mikkelson: System for capturing and modifying large pieces of genomic DNA and constructing organisms with chloroplasts. Sapphire Energy, Sapphire Energy, November 20, 2012: US08314222

The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. Also described is a method for isolating entire genomes, including chloroplast geno ...


8
Michael Mendez, Craig A Behnke, Nicole A Heaps: Variant isoprenoid producing enzymes and uses thereof. Sapphire Energy, Sapphire Energy, March 24, 2015: US08987433

This disclosure provides methods of designing and generating polypeptide variants that have altered properties compared to a parent polypeptide. The present disclosure provides methods of generating polypeptide variants, for example, variant isoprenoid synthases and/or variant prenyl transferases th ...


9
Craig A Behnke, David Molina, Soyan Lieberman, Jamie Bacher, Shuiqin Wu: Acetyl CoA carboxylases. SAPPHIRE ENERGY, Sapphire Energy, September 15, 2015: US09133489

Provided herein are novel ACCases and nucleotides encoding the same, that when introduced into a cell or organism results in an increase and/or accumulation of fatty acids, glycerol lipids, and/or oils in the cell or organism, and/or a change in the types of fatty acids, glycerol lipids, and/or oils ...


10
Christopher Yohn, April Brand, Michael Mendez, Craig A Behnke: Transformation of algae for increasing lipid production. SAPPHIRE ENERGY, Sapphire Energy, August 30, 2016: US09428779

The present disclosure provides novel proteins that when over expressed in algae result in an increase or change in fatty acid and/or glycerol lipid production and/or accumulation, without a substantial decrease in the growth rate of the alga or the break down of algal components, such as chlorophyl ...