1
Nandini Katre, Robert F Halenbeck, Robert J Goodson, Peter C McCabe, Michael J Knauf: Interleukin-2 muteins and polymer conjugation thereof. Cetus Oncology Corporation, Philip L McGarrigle, Janet E Hasak, April 27, 1993: US05206344 (225 worldwide citation)

Muteins of IL-2 in which one of the amino acids of the mature native sequence of IL-2 is replaced by a cysteine residue are prepared and conjugated through the replaced cysteine residue to a polymer selected from polyethylene glycol homopolymers or polyoxyethylated polyols, wherein said homopolymers ...


2
Danute E Nitecki: Preparation of an activated polymer ester for protein conjugation. Cetus Oncology Corporation, Grant D Green, Kenneth M Goldman, Philip L McGarrigle Jr, January 25, 1994: US05281698 (221 worldwide citation)

The present invention is a process for preparing an activated ester of polyethylene glycol or a polyoxyethylated polyol. After the activated ester is prepared, it can be reacted with a protein to form a polymer/protein conjugate. Conjugation with a polymer can reduce the protein's immunogenicity, in ...


3
Stephen P A Fodor, J Leighton Read, Lubert Stryer, Michael C Pirrung: Nucleic acid reading and analysis system. Affymetrix, Vern Norviel, Philip L McGarrigle, June 11, 2002: US06403957 (163 worldwide citation)

A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are exposed to light so as to activate the selected areas. A monomer, also containing a photoremovable group; i ...


4
Rolfe C Anderson, Robert J Lipshutz, Richard P Rava, Stephen P A Fodor: Integrated nucleic acid diagnostic device. Affymetrix, Philip L McGarrigle, William McCarthy, Ivan D Zitkovsky, December 14, 2004: US06830936 (155 worldwide citation)

The present invention provides a miniaturized integrated nucleic acid diagnostic device and system. The or more sample acquisition and preparation operations, in combination with one or more sample analysis operations. For example, the device can integrate several or all of the operations involved i ...


5
J Leighton Read, Stephen P A Fodor, Lubert Stryer, Michael C Pirrung: Support bound probes and methods of analysis using the same. Affymetrix, Philip L McGarrigle, Joseph Liebschuetz, Lisa Treannie, June 11, 2002: US06403320 (138 worldwide citation)

The present invention provides methods and apparatus for sequencing, fingerprinting and mapping biological macromolecules, typically biological polymers. The methods make use of a plurality of sequence specific recognition reagents which can also be used for classification of biological samples, and ...


6
Rolfe C Anderson, Robert J Lipshutz, Richard P Rava, Stephen P A Fodor: Method of manipulating a gas bubble in a microfluidic device. Affymetrix, Philip L McGarrigle, Alan B Sherr, Ivan D Zitkovsky, December 4, 2001: US06326211 (136 worldwide citation)

The present invention provides a miniaturized integrated nucleic acid diagnostic device and system. The device of the invention is generally capable of performing one or more sample acquisition and preparation operations, in combination with one or more sample analysis operations. For example, the d ...


7
Nandini Katre, Michael J Knauf: Solubilization of immunotoxins for pharmaceutical compositions using polymer conjugation. Cetus Corporation, Janet E Hasak, Philip L McGarrigle, Albert P Halluin, April 17, 1990: US04917888 (133 worldwide citation)

A pharmaceutical composition is prepared wherein a biologically active conjugated protein which is .beta.-interferon, interleukin-2, or an immunotoxin is dissolved in an aqueous carrier medium without the presence of a solubilizing agent. The unconjugated protein, which is not water-soluble or not r ...


8
Danute E Nitecki, Nandini Katre, Robert J Goodson, Lois Aldwin: Preparation of a polymer/interleukin-2 conjugate. Cetus Corporation, Philip L McGarrigle, Albert P Halluin, February 20, 1990: US04902502 (129 worldwide citation)

The present invention is a process for preparing a pharmaceutical composition comprising a biologically active conjugated protein. It comprises a polyethylene glycol or a polyoxyethylated polyol conjugated to IL-2. This protein is conjugated to reduce its immunogenicity, and increase it solubility, ...


9
Michael C Pirrung, Lubert Stryer, Stephen P A Fodor, J Leighton Read: Photolithographic and other means for manufacturing arrays. Affymetrix, Philip L McGarrigle Esq, Hamilton Brook Smith & Reynolds P C, Townsend Townsend and Crew, July 9, 2002: US06416952 (122 worldwide citation)

The present invention provides methods and apparatus for sequencing, fingerprinting and mapping biological macromolecules, typically biological polymers. The methods make use of a plurality of sequence specific recognition reagents which can also be used for classification of biological samples, and ...


10
Xing Su, Helin Dong, Thomas B Ryder: Amplification of nucleic acids. Affymetrix, Sandra Wells, Philip L McGarrigle, June 24, 2003: US06582938 (93 worldwide citation)

The present invention relates to the amplification of nucleic acids, preferably from mRNA. A primer and promoter are added to a target sequence to be amplified and then the target is amplified in an in vitro transcription reaction.