1
Donald I Stimpson, Julian Gordon, Joanell V Hoijer: Light scattering optical waveguide method for detecting specific binding events. Abbott Laboratories, Thomas D Brainard, Paul D Yasger, February 4, 1997: US05599668 (148 worldwide citation)

A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and ...


2
Stanley R Bouma, Omar S Khalil, Edward K Pabich: Method for detection of nucleic acid using total internal reflectance. Abbott Laboratories, Thomas D Brainard, Paul D Yasger, December 17, 1996: US05585242 (136 worldwide citation)

An apparatus and method for detecting amplified target nucleic acid is provided wherein the presence and concentration of amplified target is determined by total internal reflection over the course of the amplification reaction. A method and apparatus for detecting target nucleic acid is also provid ...


3
Stanley R Bouma, Ronald A Coules, Julian Gordon, Eric B Shain, Natalie A Solomon, Peter Zaun: Device and method for amplifying and detecting target nucleic acids. Abbott Laboratories, Thomas D Brainard, Paul D Yasger, July 8, 1997: US05645801 (117 worldwide citation)

Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then sealably mated with a detection c ...


4
Stanley R Bouma, Julian Gordon, Joanell Hoijer, Cynthia Jou, James Rhoads: Method of multiplex ligase chain reaction. Abbott Laboratories, Thomas D Brainard, Paul D Yasger, February 9, 1999: US05869252 (92 worldwide citation)

The invention relates to multiplex ligase chain reaction (LCR). Two or more purative target sequences are selected. For each one, a set of four probes is used simultaneously to amplify the putative sequence if it is present in the sample. Preferably, all the amplicons are labeled with a common label ...


5
Keith C Backman, Sheila B Bond, John J Carrino, Thomas G Laffler: Method and kits for amplifying target nucleic acids applicable to both polymerase and ligase chain reactions. Abbott Laboratories, Thomas D Brainard, Paul D Yasger, August 11, 1998: US05792607 (80 worldwide citation)

The present invention involves methods of improving LCR and PCR amplification schemes by modifying at least one probe/primer end so that the probability of the probe/prirner contributing to spurious signal development is greatly reduced. Only after specific hybridization of the modified probe/primer ...


6
Keith C Backman, John J Carrino, George H Shimer, Robert R Yocum: Ligase chain reaction with endonuclease IV correction and contamination control. Abbott Laboratories, Thomas D Brainard, Paul D Yasger, May 14, 1996: US05516663 (78 worldwide citation)

The present invention involves methods of improving the Ligase Chain Reaction (LCR.TM.) amplification schemes by modifying at least one probe end so that the probability of the probe contributing to spurious ligation and signal development is greatly reduced. Only after specific hybridization of the ...


7
Natalie A Solomon, Stanley R Bouma: Wide dynamic range nucleic acid detection using an aggregate primer series. Abbott Laboratories, Paul D Yasger, January 12, 1999: US05858732 (67 worldwide citation)

The present invention provides a method of detecting the amount of a target sequence which may be present in a test sample. The method uses an aggregate primer series, which comprises at least two primer sets, in an amplification reaction to detect the relative concentration of a target sequence whi ...


8
Peter Zaun, Stanley R Bouma, Julian Gordon, John J Kotlarik, Natalie A Solomon: Apparatus and method for transfer of a fluid sample. Abbott Laboratories, Paul D Yasger, May 30, 2000: US06068978 (66 worldwide citation)

Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a one or two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a dete ...


9
John J Carrino, Uwe Spies, Laurie A Rinehardt, Edward K Pabich: Detecting and amplifying target nucleic acids using exonucleolytic activity. Abbott Laboratories, Thomas D Brainard, Paul D Yasger, November 12, 1996: US05573907 (65 worldwide citation)

The present invention relates to improved LCR amplification schemes using at least one downstream probe modified at its 5' end to reduce or eliminate target independent amplification. The different modified probes, and kits containing them are also presented. Also presented is a method for detecting ...


10
Donald Irvine Stimpson, Julian Gordon, Joanell V Hoijer: Light scattering optical waveguide method for detecting specific binding events. Abbott Laboratories, Thomas D Brainard, Paul D Yasger, December 1, 1998: US05843651 (62 worldwide citation)

A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and ...