1
Michael C Pirrung, J Leighton Read, Stephen P A Fodor, Lubert Stryer: Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof. Affymax Technologies, Kenneth J Nussbacher, Kevin R Kaster, Vern Norviel, September 1, 1992: US05143854 (1324 worldwide citation)

Polypeptide arrays can be synthesized on a substrate by attaching photoremovable groups to the surface of a substrate, exposing selected regions of the substrate to light to activate those regions, attaching an amino acid monomer with a photoremovable group to the activated regions, and repeating th ...


2
Kary B Mullis, Henry A Erlich, David H Gelfand, Glenn Horn, Randall K Saiki: Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme. Cetus Corporation, Kevin R Kaster, Janet E Hasak, Albert P Halloin, October 23, 1990: US04965188 (1294 worldwide citation)

A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary ...


3
Kary B Mullis, Henry A Erlich, Norman Arnheim, Glenn T Horn, Randall K Saiki, Stephen J Scharf: Process for amplifying, detecting, and/or cloning nucleic acid sequences. Cetus Corporation, Kevin R Kaster, Janet E Hasak, Albert P Halluin, January 24, 1989: US04800159 (1080 worldwide citation)

The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending ...


4
Stephen P A Fodor, Lubert Stryer, Michael C Pirrung, J Leighton Read: Very large scale immobilized polymer synthesis. Affymax Technologies, Kevin R Kaster, Vern Norviel, June 13, 1995: US05424186 (506 worldwide citation)

A method for synthesizing oligonucleotides on a solid substrate. The method provides for the irradiation of a first predefined region of the substrate without irradiation of a first predefined region of the substrate. The irradiation of a second predefined region of the substrate. The irradiation st ...


5
David H Gelfand, Susanne Stoffel, Frances C Lawyer, Randall K Saiki: Purified thermostable enzyme. Cetus Corporation, Janet E Hasak, Kevin R Kaster, Albert P Halluin, December 26, 1989: US04889818 (418 worldwide citation)

A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling ch ...


6
James L Winkler, Stephen P A Fodor, Christopher J Buchko, Debra A Ross, Lois Aldwin: Very large scale immobilized polymer synthesis using mechanically directed flow paths. Affymax Technologies, Kevin R Kaster, Vern Norviel, January 24, 1995: US05384261 (375 worldwide citation)

A method and device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are flowed through the channels, the substrate is rotated by a rotatin ...


7
Michael C Pirrung, J Leighton Read, Stephen P A Fodor, Lubert Stryer: Large scale photolithographic solid phase synthesis of an array of polymers. Affymax Technologies, Kevin R Kaster, Vern Norviel, April 11, 1995: US05405783 (298 worldwide citation)

A technique for the synthesis of arrays of diverse polymers such as polypeptides and nucleic acids. The technique beneficially utilizes solid-phase chemistry techniques. Preferred embodiments utilize photolabile protecting groups, and photolithography. The technique forms polymers with monomer seque ...


8
Ronald W Barrett, Michael C Pirrung, Lubert Stryer, Christopher P Holmes, Steven A Sundberg: Spatially-addressable immobilization of anti-ligands on surfaces. Affymax Technologies, Kevin R Kaster, Jeffrey K Weaver, Karen B Dow, October 12, 1993: US05252743 (285 worldwide citation)

Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise ...


9
Larry J Johnson, Joseph T Widunas: Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps. Cetus Corporation, Ronald C Fish, Kevin R Kaster, August 13, 1991: US05038852 (208 worldwide citation)

There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing ...


10
Peter J Schatz, Millard G Cull, Jeff F Miller: Peptide library and screening method. Affymax Technologies, Kevin R Kaster, Kenneth J Nussbacher, December 14, 1993: US05270170 (184 worldwide citation)

A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a DNA binding protein and a random peptide and also encode a binding site for the DNA binding protein can be used to screen for novel ligands. The screen ...



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