1
Norman M Whiteley, Michael W Hunkapiller, Alexander N Glazer: Detection of specific sequences in nucleic acids. Applied Biosystems, Joseph H Smith, November 28, 1989: US04883750 (319 worldwide citation)

The invention provides a method for diagnosis of genetic abnormalities or other genetic conditions which can be readily automated. The method is used to determine the presence or absence of a target sequence in a sample of denatured nucleic acid and entails hybridizing the sample with a probe comple ...


2
G Richard Cathcart, Thomas Brennan Marquez, John A Bridgham, George S Golda, Harry A Guiremand, Marianne Hane, Louis B Hoff, Eric Lachenmeier, Melvyn N Kronick, Douglas H Keith, Paul E Mayrand, Michael L Metzker, William J Mordan, Lincoln J McBride, John Shigeura, Chen Hanson Ting, Norman M Whiteley: Automated molecular biology laboratory. Perkin Elmer Applied Biosystems Division, Donald R Boys, Joseph H Smith, August 22, 1995: US05443791 (318 worldwide citation)

A liquid-handling instrument has a worksurface with registration for modular stations to support containers of liquid, pipette apparatus with a pipette tip coupled to a sensing circuit, a robotic translation system for moving the pipette tip, and a control system with an iconic user interface for pr ...


3
John L Vaught, Frank L Cloutier, David K Donald, John D Meyer, Christopher A Tacklind, Howard H Taub: Thermal ink jet printer. Hewlett Packard Company, Douglas A Kundrat, Joseph H Smith, December 25, 1984: US04490728 (277 worldwide citation)

A thermal ink jet printer is disclosed in which ink droplets are ejected from an orifice by the explosive formation of a vapor bubble within the ink supply due to the application of a two part electrical pulse to a resistor within the ink supply. The electrical pulse comprises a precurser pulse and ...


4
Steven M Menchen, Linda G Lee, Charles R Connell, N Davis Hershey, Vergine Chakerian, Sam Woo, Steven Fung: 4,7-dichlorofluorescein dyes as molecular probes. Applied Biosystems, Stephen C Macevicz, Joseph H Smith, February 23, 1993: US05188934 (231 worldwide citation)

Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 1',2',7',8'-dibenzo-4,7-dichlorofluoresceins. Methods of using the dyes in automated DNA sequencing are describe ...


5
Steven Fung, Sam L Woo, Lloyd M Smith: Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof. Applied Biosystems Incorporated, Joseph H Smith, July 12, 1988: US04757141 (176 worldwide citation)

The compounds of the invention include novel linking agents comprising 2-substituted-3-protected-1,3,2-oxazaphosphacycloalkanes and their phosphoramidite precursors. The compounds of the invention further include conjugates of the above linking agents with oligonucleotides and polymer supports. The ...


6
Michael W Hunkapiller, Charles R Connell, William J Mordan, John D Lytle, John A Bridgham: Real time scanning electrophoresis apparatus for DNA sequencing. Applied Biosystems, Joseph H Smith, March 7, 1989: US04811218 (172 worldwide citation)

A real-time, automated, nucleic acid sequencing apparatus that offers high speed, definitive sequencing on many samples at the same time. The apparatus permits more than one clone to be sequenced at a time, thus vastly decreasing the time required to sequence longer fragments and reducing sequencing ...


7
Edward J McInerney, E Ronald Dornseif, Norman E Zetterquist: Interlayer dielectric process. Genus, Joseph H Smith, September 1, 1987: US04690746 (167 worldwide citation)

A method for producing a film over a topologically non-planar surface of a material which has a sputter etch rate which is higher in a direction parallel to the plane of the wafer than in a direction perpendicular to the plane of the wafer. Key steps in the process include first, depositing the mate ...


8
Steven Fung, Sam L Woo, Richard P Haugland, Steven M Menchen, Charles R Connell: Method of detecting electrophoretically separated oligonucleotides. Applied Biosystems, Steven C Macevicz, Joseph H Smith, August 8, 1989: US04855225 (162 worldwide citation)

A method is provided for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis. The method entails labeling members of each class of oligonucleotide with dyes selected from separate sets of dyes so that members of the same class are labeled with dyes from ...


9
William P Kruger, John L Vaught: Ink jet printer with bubble driven flexible membrane. Hewlett Packard Company, Douglas A Kundrat, Joseph H Smith, October 30, 1984: US04480259 (151 worldwide citation)

An apparatus is disclosed for propelling ink droplets from an ink jet nozzle which uses an expanding bubble as a driving mechanism. Unlike other thermal ink jet devices, the ink itself is not used to provide the driving bubble. Rather a two fluid system is disclosed whereby a flexible membrane is us ...


10
Norman M Whiteley, Michael W Hunkapiller, Alexander N Glazer: Detection of specific sequences in nucleic acids. Applied Biosystems, Joseph H Smith, Peter Dehlinger, September 7, 1993: US05242794 (149 worldwide citation)

The invention provides a method for diagnosis of genetic abnormalities or other genetic conditions which can be readily automated. The method is used to determine the presence or absence of a target sequence in a sample of denatured nucleic acid and entails hybridizing the sample with a probe comple ...