1
George Fanta, Patricia J Slininger: Enzyme immobilization with a hydrolyzed polysaccharide graft copolymer. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, John D Fado, Margaret A Connor, July 4, 1989: US04845035 (127 worldwide citation)

Immobilized enzyme-polysaccharide graft copolymer products are prepared by hydrolyzing a polysaccharide graft copolymer and then contacting the hydrolyzed copolymer with an enzyme to immobilize the enzyme on the copolymer. The copolymer-enzyme products retain large quantities of active enzyme, and a ...


2
Lawrence A Johnson: Method to preselect the sex of offspring. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, John D Fado, Curtis P Ribando, August 4, 1992: US05135759 (118 worldwide citation)

Intact X and Y chromosome-bearing sperm populations of rabbits and swine were separated according to DNA content using a flow cytometer/cell sorter. Sperm viability was maintained by special staining techniques and by sorting and collecting the sperm in nutrient media. The sorted sperm were surgical ...


3
Willem Rens, Glenn R Welch, Lawrence A Johnson: Flow cytometry nozzle for high efficiency cell sorting. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, Curtis P Ribando, John D Fado, November 16, 1999: US05985216 (113 worldwide citation)

A sorting nozzle having two tapered zones with elliptical cross-sections is able to orient and sort a large fraction of asymmetrical or "flattened" cells. This nozzle has particular application for sorting viable male (Y) and female (X) sperm populations in a cell sorter.


4
Alice A Christen, Donna M Gibson, John Bland: Production of taxol or taxol-like compounds in cell culture. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, John D Fado, Curtis P Ribando, May 28, 1991: US05019504 (73 worldwide citation)

Tissue of Taxus brevifolia has been successfully cultured to produce taxol, related alkaloids, and alkaloid precursors. These procedures will provide a supply of chemotherapeutic agents.


5
Mary J Kretsch, Moira A Gunn, Alice K Fong: Method and system for measurement of intake of foods, nutrients and other food components in the diet. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, John D Fado, Margaret A Connor, August 3, 1993: US05233520 (69 worldwide citation)

An interactive computerized dietary measurement system and process which can be used by lay people for accurate measurement of the intake of foods, nutrients, and other food components in the diet. The system includes a computer device coupled with an electronic balance, output/display device(s), us ...


6
Karin D E Everett, Arthur A Andersen: Intergenic spacer target sequence for detecting and distinguishing Chlamydial species or strains. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, Curtis P Ribando, John D Fado, July 17, 2001: US06261769 (67 worldwide citation)

The intergenic spacer between the 16S and the 23S rRNA genes and a 131-bp segment at the 3′ end of Domain I of the 23S gene provides suitable target sequences for developing probes and primers useful in detecting and identifying Chlamydiaceae in laboratory and clinical samples.


7
Clark M Welch, Bethlehem K Andrews: Catalysts and processes for formaldehyde-free durable press finishing of cotton textiles with polycarboxylic acids. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, John D Fado, April 11, 1989: US04820307 (63 worldwide citation)

Catalysts for the rapid esterification and crosslinking of fibrous cellulose in textile form by polycarboxylic acids at elevated temperatures are disclosed. The catalysts are acidic or weakly basic salts selected from the alkali metal dihydrogen phosphates and alkali metal salts of phosphorous, hypo ...


8
Lyle M Carter: Pressure and depth control in agricultural implement. The United States of America represnted by the Secretary of Agriculture, M Howard Silverstein, John D Fado, August 10, 1993: US05234060 (61 worldwide citation)

A soil contacting implement and depth gauging wheel at a predetermined vertical distance from the implement are provided for attachment to a tractor. Upward or downward movement of the wheel on the soil causes a deviation from the predetermined vertical displacement thereby triggering an adjustment ...


9
Robert B Hespell, Herbert A Wyckoff, Bruce S Dien, Rodney J Bothast: Stabilization of pet operon plasmids and ethanol production in bacterial strains lacking lactate dehydrogenase and pyruvate formate lyase activities. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, Randall E Deck, John D Fado, August 28, 2001: US06280986 (60 worldwide citation)

Recombinant bacteria are disclosed which are transformed with heterologous DNA coding for alcohol dehydrogenase (adh) and pyruvate decarboxylase (pdc), and which are effective for use in the production of ethanol, but which do not require the presence of antibiotics in the culture medium to maintain ...


10
Terry A Isbell, Thomas P Abbott, Svajus Asadauskas, Joseph E Lohr Jr: Biodegradable oleic estolide ester base stocks and lubricants. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, Curtis P Ribando, John D Fado, January 25, 2000: US06018063 (59 worldwide citation)

Esters of estolides derived from oleic acids are characterized by superior properties for use as lubricant base stocks. These estolides may also be used as lubricants without the need for fortifying additives normally required to improve the lubricating properties of base stocks.



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