31
Margaret Moreland, Danute E Nitecki: Solubilization of proteins for pharmaceutical compositions using heparin fragments. Cetus Corporation, Janet E Hasak, Albert P Halluin, May 17, 1988: US04745180 (54 worldwide citation)

A pharmaceutical composition is prepared wherein a biologically active conjugated protein which is .beta.-interferon, interleukin-2, or an immunotoxin is dissolved in an aqueous carrier medium without the presence of a solubilizing agent. The unconjugated protein, which is poorly or not at all water ...


32
R Glenn Hammonds Jr, Anthony J Mason: Mammalian expression of the bone morphogenetic protein-2B using BMP2A/BMP2B fusion. Genentech, Janet E Hasak, December 1, 1992: US05168050 (54 worldwide citation)

A DNA construct is provided comprising DNA encoding a mature BMP-2 upstream of which is DNA encoding a precursor portion of a mammalian protein other than the BMP-2. Also provided are mammalian expression vectors and hosts containing such a DNA construct and methods for improved expression using suc ...


33
Ross G Clark, Michael J Cronin: Method of enhancing growth in patients using combination therapy. Genentech, Janet E Hasak, June 30, 1992: US05126324 (54 worldwide citation)

A method is disclosed for enhancing growth of a mammal by administration of a combination of effective amounts of IGF-I and GH so as to enhance the growth of the mammal over the enhancement in growth achieved using either IGF-I or GH alone in an amount equal to that used for either IGF-I or GH, resp ...


34
Ze ev Shaked, Sidney N Wolfe: Controlled oxidation of microbially produced cysteine-containing proteins. Cetus Corporation, Albert P Halluin, Janet E Hasak, July 23, 1985: US04530787 (52 worldwide citation)

Method of oxidizing reduced cysteine-containing microbially produced synthetic proteins, such as synthetic IFN-.beta. or synthetic IL-2, in a controlled manner so that the synthetic proteins have the same disulfide bridging as their native counterparts. The oxidation employs o-iodosobenzoate as oxid ...


35
David J Waugh: Lightweight rear-wheel bicycle suspension. Henri Charmasson, Janet E Hasak, April 15, 1986: US04582343 (50 worldwide citation)

A lightweight rear-wheel suspension for a bicycle which includes a triangulated main frame, a rear wheel support frame such as a rear fork rotatably supporting a rear wheel and mounted to the main frame so that it pivots about the foot crank rotational axis, providing upward and downward movement re ...


36
Edward L Sheldon III, Corey H Levenson, Kary B Mullis, Henry Rapoport, Robert M Watson: Process for labeling nucleic acids and hybridization probes. Cetus Corporation, Albert P Halluin, Janet E Hasak, October 14, 1986: US04617261 (48 worldwide citation)

Nucleic acids may be labeled by intercalating the alkylating intercalation moiety of a labeling reagent into a partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid ...


37
Anthony J Mason, Peter H Seeburg: Nucleic acid encoding the ba chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid. Genentech, Janet E Hasak, June 1, 1993: US05215893 (47 worldwide citation)

DNA encoding the prepro inhibin .alpha. and .beta. chains has been isolated. This DNA is ligated into expression vectors and used to transform host cells for the preparation of inhibin or activin. Also provided are prohormone domains and other inhibin .alpha. and .beta. chain derivatives having ther ...


38
Michael W Konrad, Leo S Lin: Process for recovering human IFN-.beta. from a transformed microorganism. Cetus Corporation, Albert P Halluin, Thomas E Ciotti, Janet E Hasak, May 22, 1984: US04450103 (47 worldwide citation)

A process for recovering IFN-.beta. from transformed bacteria comprising: disrupting the cell membranes of the bacteria; solubilizing the IFN-.beta. from the disruptate into an aqueous medium with a solubilizing agent such as sodium dodecyl sulfate; extracting the IFN-.beta. from the aqueous medium ...


39
Kirston E Koths, Robert F Halenbeck: Method for promoting disulfide bond formation in recombinant proteins. Cetus Corporation, Albert P Halluin, Janet E Hasak, February 25, 1986: US04572798 (46 worldwide citation)

Reduced cysteine-containing proteins consisting of recombinant IFN-.beta., IL-2 or muteins thereof may be oxidized selectively so that the recombinant proteins have essentially the same disulfide bridging and biological activity as their native counterparts. The oxidized product is substantially fre ...


40
Chung C Hsu, Hoc M Nguyen, Sylvia S Wu: Reconstitutable lyophilized protein formulation. Genentech, Janet E Hasak, March 9, 1993: US05192743 (45 worldwide citation)

A formulation for proteins is provided that comprises a lyophilized mixture of a protein, a water-soluble etherified cellulose in an amount that upon reconstitution of the formulation will be sufficient to form a gel, and an excipient to facilitate rehydration of the gel and to maintain protein inte ...