1
David H Gelfand, Pamela M Holland, Randall K Saiki, Robert M Watson: Homogeneous assay system using the nuclease activity of a nucleic acid polymerase. Hoffman La Roche, George M Gould, Dennis P Tramaloni, Stacev R Sias, May 11, 1993: US05210015 (592 worldwide citation)

The present invention is directed to a process of detecting a target nucleic acid using labeled oligonucleotides. This process uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide frag ...


2
David H Gelfand, Pamela M Holland, Randall K Saiki, Robert M Watson: Nucleic acid detection by the 5-3exonuclease activity of polymerases acting on adjacently hybridized oligonucleotides. Hoffmann La Roche, George M Gould, Dennis P Tramaloni, Stacey R Sias, January 30, 1996: US05487972 (250 worldwide citation)

A process of detecting a target nucleic acid using labeled oligonucleotides which uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and thus releasing labeled oligonucleotide fragments for detection. This process is e ...


3
Robert Karasiewicz, Carlo Nalin, Perry Rosen: Peg-interferon conjugates. Hoffmann La Roche, George M Gould, William H Epstein, Catherine R Roseman, January 17, 1995: US05382657 (204 worldwide citation)

Substituted PEG-interferon conjugates of formulae IA and IB where PEG is linked by means of activated linking reagents of formulae IIA, IIB, or IIB-1 to an amino group in the interferon, and activated linking reagents of formulae IIA, IIB, or IIB-1. The conjugates are not readily susceptible to in v ...


4
David H Gelfand: Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription. Hoffman LaRoche, George M Gould, Dennis P Tramaloni, Stacey RG351 5 Sias, June 21, 1994: US05322770 (184 worldwide citation)

Methods are provided for the replication and amplification of RNA sequences by thermostable DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 92 kDa Taq, 64 kDa Taq, and Tth DNA polymerase. Reverse transcription is coupled to PCR amplification in a one enzyme procedure usin ...


5
Peter Loeliger: Stilbene derivatives. Hoffmann La Roche, Jon S Saxe, George M Gould, William H Epstein, April 20, 1982: US04326055 (175 worldwide citation)

This invention is directed to 5,6,7,8-tetrahydro-naphthyl or indanyl stilbene derivatives which are useful as tumor inhibiting agents, in the treatment of neoplasms, dermatological conditions and rheumatic illnesses.


6
Christof Fattinger: Microoptical sensor and method. Hoffmann La Roche, George M Gould, George W Johnston, October 3, 1995: US05455178 (166 worldwide citation)

A microoptical sensor and a corresponding method for detecting chemical substances in a sample. The sample is brought into contact with the waveguiding layer of a planar optical waveguide. Coherent light is coupled into the waveguiding layer, propagates therein as a guided wave, and the latter is de ...


7
Alice M Wang, Michael V Doyle, David F Mark: Quantitation of nucleic acids using the polymerase chain reaction. Hoffmann LaRoche, George M Gould, Dennis P Tramaloni, Stacey R Sias, June 15, 1993: US05219727 (165 worldwide citation)

The present invention provides a method for determining the amount of a target acid segment in a sample by polymerase chain reaction. The method involves the simultaneous amplification of the target nucleic acid segment and an internal standard nucleic acid segment. The amount of amplified DNA from ...


8
Heini Ernst, Albert Guenin: Automatic regulation of respirators. Hoffmann La Roche, Samuel L Welt, George M Gould, Mark L Hopkins, June 8, 1976: US03961627 (161 worldwide citation)

Regulation of the flow and pressure of the respiration gas in a respirator coupled to a patient is disclosed in regard to a respirator arrangement having a flow and pressure measuring device arranged proximate the patient connection for determining the flow and pressure of the respiration gas and co ...


9
Mikhail I Barnik, Sergey V Belyaev, Jurg Funfschilling, Nikolai Malimonenko, Martin Schadt, Klaus Schmitt: Polarizer device. Hoffmann La Roche, NIOPIC Moscow Research and Production Association, George M Gould, George W Johnston, August 10, 1993: US05235443 (158 worldwide citation)

The polarizer comprises a layer of cholesteric liquid crystal with Grandjean structure disposed in the path of the light and in which the light in a given wavelength range is reflected with selective and circular polarization or is transmitted with reversed direction of rotation, and a mirror which ...


10
Alice M Wang, Michael V Doyle, David F Mark: Quantitation of nucleic acids using the polymerase chain reaction. Hoffmann La Roche, George M Gould, Dennis P Tramaloni, Stacey R Sias, December 19, 1995: US05476774 (137 worldwide citation)

The present invention provides a method for determining the amount of a target acid segment in a sample by polymerase chain reaction. The method involves the simultaneous amplification or the target nucleic acid segment and an internal standard nucleic acid segment. The amount of amplified DNA from ...



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