1
Philip L Felgner, Jon A Wolff, Gary H Rhodes, Robert W Malone, Dennis A Carson: Delivery of exogenous DNA sequences in a mammal. VICAL Incorporated, Wisconsin Alumni Research Foundation, Knobbe Martens Olson & Bear, December 3, 1996: US05580859 (762 worldwide citation)

Polynucleotide sequences, comprising DNA and RNA molecules can be directly administered, for example by injection, to tissues, such as muscle, and expressed as a protein, polypeptide or polypeptide. The polynucleotides can be contained within liposomes or the polynucleotides can free from associatio ...


2
Philip L Felgner, Jon A Wolff, Gary H Rhodes, Robert W Malone, Dennis A Carson: Induction of a protective immune response in a mammal by injecting a DNA sequence. Vical Incorporated, Wisconsin Alumni Research Foundation, Knobbe Martens Olson & Bear, December 31, 1996: US05589466 (546 worldwide citation)

A method for delivering an isolated polynucleotide such as DNA or RNA, to the interior of a cell in a mammal comprising the injection of an isolated polynucleotide into a muscle of the mammal where the polynucleotide is taken up by the cells of the muscle and exerts a therapeutic effect on the mamma ...


3
Philip L Felgner, Jon Asher Wolff, Gary H Rhodes, Robert Wallace Malone, Dennis A Carson: Induction of a protective immune response in a mammal by injecting a DNA sequence. Vical Incorporated, Sterne Kesseler Goldstein & Fox P L L C, April 10, 2001: US06214804 (273 worldwide citation)

A method for delivering an isolated polynucleotide to the interior of a cell in a vertebrate, comprising the interstitial introduction of an isolated polynucleotide into a tissue of the vertebrate where the polynucleotide is taken up by the cells of the tissue and exerts a therapeutic effect on the ...


4
Philip L Felgner, Jon Asher Wolff, Gary H Rhodes, Robert Wallace Malone, Dennis A Carson: Generation of antibodies through lipid mediated DNA delivery. Wisconsin Alumni Research Foundation, Vical Incorporated, Knobbe Martens Olson & Bear, December 30, 1997: US05703055 (253 worldwide citation)

A method is disclosed for delivering an isolated polynucleotide to the interior of a cell in a vertebrate, comprising the interstitial introduction of an isolated polynucleotide into a tissue of the vertebrate where the polynucleotide is taken up by the cells of the tissue and exerts a therapeutic e ...


5
Jon A Wolff, David J Duke, Philip L Felgner: Expression of exogenous polynucleotide sequences cardiac muscle of a mammal. Vical Incorporated, Wisconsin Alumni Research Foundation, Knobbe Martens Olson & Bear, December 2, 1997: US05693622 (172 worldwide citation)

The present invention provides a method for delivering a pharmaceutical polypeptide to the interior of a cardiac cell of a vertebrate in vivo, comprising the step of introducing a preparation comprising a pharmaceutically acceptable injectable carrier and naked polynucleotide operatively coding for ...


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7
Holly Horton, Suezanne Parker, Marston Manthorpe, Philip L Felgner, Jukka Hartikka: Methods for treating cancer using cytokine-expressing polynucleotides. Vical Incorporated, Sterne Kessler Goldstein & Fox P L L C, September 11, 2007: US07268120 (49 worldwide citation)

The present invention provides a pharmaceutical composition, comprising a non-infectious, non-integrating polynucleotide construct comprising a polynucleotide encoding an interferon ω and one or more cationic compounds. The present invention also provides methods of treating cancer in a mammal, comp ...


8
Gary J Nabel, Elizabeth G Nabel, Denise Lew, Magda Marquet: Plasmids suitable for gene therapy. Vical Incorporated, Knobbe Martens Olson & Bear, June 8, 1999: US05910488 (49 worldwide citation)

The invention provides vectors adapted for use in transferring into tissue or cells of an organism genetic material encoding one or more cistrons capable of expressing one or more immunogenic or therapeutic peptides and related methods.


9
Carl Wheeler: Cytofectin dimers and methods of use thereof. Vical Incorporated, Sterne Kessler Goldstein & Fox P L L C, February 24, 2004: US06696424 (45 worldwide citation)

A composition is provided comprising a novel cationic lipid compound having hydrophobic tails and two quaternary ammonium headgroups bridged by a linker. The composition is useful as a cytofectin for facilitating delivery and transfection of biologically active agents, particularly anionic bioactive ...


10
Carl J Wheeler: Adjuvant compositions and methods for enhancing immune responses to polynucleotide-based vaccines. Vical Incorporated, Sterne Kessler Goldstein & Fox P L L C, July 1, 2003: US06586409 (41 worldwide citation)

The invention provides adjuvants, immunogenic compositions, and methods useful for polynucleotide-based vaccination and immune response. In particular, the invention provides an adjuvant of cytofectin:co-lipid mixture wherein cytofectin is GAP-DMORIE.