1
Garfield P Royer: Modifying enzymes with polyethylene glycol and product produced thereby. Pierce Chemical Company, January 11, 1977: US04002531 (214 worldwide citation)

A process is disclosed for the preparation of the monoalkyl polyethyleneglycol derivatives of enzymes containing free amino groups. The process involves reductively alkylating the free amino groups of the enzyme with the aldehyde derivative of a monoalkyl polyethyleneglycol.


2
Carl R Clark: Apparatus for rapid immunoassays. Pierce Chemical Company, June 15, 1993: US05219528 (55 worldwide citation)

An apparatus for use in a ligand-receptor assay procedure for the quantitative determination of the concentration of a target ligand in a liquid sample. The apparatus contains top and middle plates having holes therethrough. The holes in the two plates are in axial alignment and the holes in the mid ...


3
Carl Clark: Dialysis device with hermetically sealed vacant chamber. Pierce Chemical Company, April 2, 1996: US05503741 (29 worldwide citation)

A device for the dialysis of a sample. The device embodies a hermetically sealed sample chamber formed by a gasket with dialysis membranes affixed to each side in substantially parallel relationship. The gasket is impermeable to the sample being dialyzed, but is penetrable and reusable such that a n ...


4
Edward K Fujimoto: Biotin containing heterobifunctional cleavable compounds. Pierce Chemical Company, July 2, 1996: US05532379 (23 worldwide citation)

Trifunctional cross-linking compounds are disclosed. The compounds contain the biotin moiety, the NHS active ester, and a photoactivatable aryl azide. The presence of a disulfide bond in association with either the ester or azide permits the compound to be cleaved.


5
Gindler E Melvin: Colorimetric determination of calcium in biologic fluids. Pierce Chemical Company, August 28, 1973: US3754865 (21 worldwide citation)

Methylthymol blue dye in colorimetrically analyzing a biologic fluid such as blood serum for calcium content. Analysis can be accomplished in the presence of proteins, phosphates and bilirubin. 8-Quinolinol can be used to bind magnesium without an accompanying adverse effect on the measurement of ca ...


6
A Krishna Mallia: Rapid assays for protein kinase activity. Pierce Chemical Company, June 18, 1996: US05527688 (20 worldwide citation)

A method of measuring enzymatic activity of a protein kinase is disclosed. The method is an improvement to existing methodology which involves phosphorylating a peptide substrate, adsorbing the phosphorylated peptide to a solid phase, washing the phase to remove non-adsorbed constituents, and measur ...


7
Garfield P Royer: Molecular transformation procedure. Pierce Chemical Company, February 9, 1982: US04315074 (17 worldwide citation)

A process is disclosed for synthesizing a peptide chain involving reacting, in aqueous solution, an amino acid containing a blocked alpha amino or carboxyl group with a polynucleotide handle to form a covalently bonded complex. The complex is separated from unreacted acid by reversible coupling to a ...


8
Garfield P Royer: Production of polypeptides using polynucleotides. Pierce Chemical Company, January 8, 1980: US04182654 (15 worldwide citation)

A process is disclosed for synthesizing a peptide chain involving reacting, in aqueous solution, an amino acid containing a blocked alpha amino or carboxyl group with a polynucleotide handle to form a covalently bonded complex. The complex is separated from unreacted acid by reversible coupling to a ...


9
Gindler E Melvin: Method and composition for the determination of albumin. Pierce Chemical Company, Wolfe Hubbard Leydig Voit & Osann, May 20, 1975: US3884637 (14 worldwide citation)

A working solution and method for the spectrophotometric determination of albumin, employing a sulphonphthalein dye such as bromcresol green and a phosphate-containing anionic surfactant to prevent precipitation of protein-dye complexes.


10
Paul D Davis, Kelli D Feather Henigan, Kimberly Hines: Assay of peroxidase activity. Pierce Chemical Company, Mark A Litman & Assoc P A, August 13, 2002: US06432662 (14 worldwide citation)

Chemiluminescent detection of molecules of synthetic or natural origin such as proteins and nucleic acids (DNA and RNA), as well as other biologic molecules, is increasingly replacing radioactive detection as the method of choice where sensitivity is critical. In such assays, luminescence is customa ...