1
William Frank Martin, Ruediger Hain, Klaus Guenther Tietjen, Marco Busch, Andreas S Kloti: Method of determining activity of 1-deoxy-D-xylulose-5-phosphate reductoisomerase and 1-deoxy-D-xylulose-5-phosphate synthase. Paradigm Genetics R T P, Bayer, Joseph T Majka, Elaine T Sale, Sorojini J Biswas, October 16, 2001: US06303365 (31 worldwide citation)

The invention relates to DNA which encodes Arabidopsis 1-deoxy-xylulose-5-phosphate reductoisomerase and to a method of identifying modulators of the 1-deoxy-D-xylulose-5-phosphate reductoisomerase and 1-deoxy-D-xylulose-5-phosphate synthase activity.


2
Jeffrey Shuster, Matthew M Tanzer, Lisbeth Hamer, Kiichi Adachi, Todd M DeZwaan, Sze Chung Lo, Maria Victoria Montenegro Chamorro, Blaise Darveaux, Sheryl Frank, Ryan Heiniger, Sanjoy K Mahanty, Huaqin Pan, Amy Skalchunes, Rex W Tarpey: Methods for the identification of inhibitors of homocitrate synthase as antibiotics. Paradigm Genetics, Deborah H Spencer, Timothy G Hofmeyer, Laura L Kiefer, October 14, 2003: US06632631 (12 worldwide citation)

The present inventors have discovered that homocitrate synthase is essential for fungal pathogenicity. Specifically, the inhibition of homocitrate synthase gene expression in fungi results in no signs of successful infection or lesions. Thus, homocitrate synthase can be used as a target for the iden ...


3
John W Rice, Andreas S Kloti, John M Crawford Jr, Beth Lanning, Sandy J Stewart: Methods for determining deoxyxylulose 5-phosphate synthase activity. Paradigm Genetics, Elaine T Sale, Joseph T Majka, Henry Nowak, December 4, 2001: US06326164 (11 worldwide citation)

The invention is directed to methods and compositions for the determination of deoxyxylulose 5-phosphate synthase (DXPS) activity. The methods and compositions of the invention are amenable to high-throughput screening assays for the identification of inhibitors and enhancers of DXPS activity. Such ...


4
Douglas C Boyes: Spray booth for reproducible application of agrichemicals. Paradigm Genetics, Joseph T Majka, Henry P Nowak, March 26, 2002: US06360482 (8 worldwide citation)

A spray booth is provided for reproducible application of agrichemicals to plants. The spray booth comprises a support member for supporting a plurality of plants, a cover having a plurality of sides and a top and forming an enclosure with the support member for enclosing the plants therein, an agri ...


5
Carol Hamilton: High throughput method, apparatus and kit for plating microorganisms and cell cultures. Paradigm Genetics, Deborah H Spencer, Timothy G Hofmeyer, Laura L Kiefer, December 9, 2003: US06660519 (6 worldwide citation)

The present invention relates to a highly effective method, apparatus and kit for growing and plating out cultures of microorganisms or cell culture, especially derived from culture blocks containing multiple wells, such as a 96-well or 384-well plate or block.


6
Todd DeZwaan, Sze Chung Lo, Maria Victoria Montenegro Chamarro, Sheryl Frank, Blaise Darveaux, Sanjoy K Mahanty, Ryan Heiniger, Amy Skalchunes, Huaqin Pan, Rex Tarpey, Jeffrey Shuster, Matthew M Tanzer, Lisbeth Hamer, Kiichi Adachi: Methods for the identification of inhibitors of 5-aminolevulinate synthase as antibiotics. Paradigm Genetics, Laura L Kiefer, Timothy G Hofmeyer, Deborah H Spencer, February 10, 2004: US06689578 (5 worldwide citation)

The present inventors have discovered that 5-Aminolevulinate synthase is essential for fungal pathogenicity. Specifically, the inhibition of 5-Aminolevulinate synthase gene expression in fungi results in no signs of successful infection or lesions. Thus, 5-Aminolevulinate synthase can be used as a t ...


7
John Rice, Beth Lanning, John Crawford, Gordon Nye: Methods for measuring cysteine and determining cysteine synthase activity. Paradigm Genetics, Laura L Kiefer, Timothy G Hofmeyer, Deborah H Spencer, August 12, 2003: US06605459 (4 worldwide citation)

Assays, preferably high throughput assays, for determining cysteine concentration, cysteine synthase activity, and identifying herbicides, fungicides, bactericides, and insecticides. Cysteine concentration is quantitated by contacting cysteine with a coumarin dye capable of conjugating with cysteine ...


8
Kiichi Adachi, John E Hamer, Lisbeth Hamer: Methods and materials for the rapid and high volume production of a gene knock-out library in an organism. Paradigm Genetics, Laura L Kiefer, Timothy G Hofmeyer, Deborah H Spencer, May 13, 2003: US06562624 (3 worldwide citation)

The present invention relates to methods for facilitating site directed homologous recombination in a eukaryotic organism to produce genomic mutants using transposon mediated mutagenesis of cosmid vectors carrying large genomic inserts from the target eukaryotic organism. The transposon carries a bi ...


9
Andreas S Klöti, Rao Mulpuri: Method for transforming plants. Paradigm Genetics, Joseph T Majka, Henry P Nowak, March 5, 2002: US06353155 (3 worldwide citation)

A method for preparing transgenic plants and seeds is claimed. The method is particularly useful for high-throughput transformation of plants, such as


10
Kloti Andreas S: Methods and apparatus for transformation of monocotyledenous plants using agrobacterium in combination with vacuum filtration. Paradigm Genetics, Kloti Andreas S, MAJKA Joseph T, February 22, 2001: WO/2001/012828 (3 worldwide citation)

A method for transforming monocotyledenous plants such as rice, wheat, rice, corn, barley, sorghum, millet, teff, rye, and oats involves contacting the flowers of the monocotyledenous plant with an Agrobacterium solution or suspension, and subjecting the plant to a vacuum such that the Agrobacterium ...