1
Robert C Ladner, Robert E Bird, Karl Hardman: Single polypeptide chain binding molecules. Genex Corporation, Saidman Sterne Kessler & Goldstein, August 7, 1990: US04946778 (1664 worldwide citation)

The invention pertains to a single polypeptide chain binding molecule which has binding specificity and affinity substantially similar to the binding specificity and affinity of the light and heavy chain aggregate variable region of an antibody, to genetic sequences coding therefor, and to recombina ...


2
Robert C Ladner: Computer based system and method for determining and displaying possible chemical structures for converting double- or multiple-chain polypeptides to single-chain polypeptides. Genex Corporation, Saidman Sterne Kessler & Goldstein, November 14, 1989: US04881175 (100 worldwide citation)

A computer based system and method determines and displays possible chemical structures for converting two naturally aggregated but chemically separated polypeptide chains into a single polypeptide chain which will fold into a three dimensional structure very similar to the original structure made o ...


3
Wayne E Swann: Vermiculite as a carrier support for immobilized biological materials. Genex Corporation, Bernard Rothwell & Brown, March 12, 1985: US04504582 (75 worldwide citation)

Biological materials are immobilized by being absorbed into vermiculite particles which then are coated with a polymeric coating material. A variety of cross-linking, condensing, and gelling agents may be used to strengthen and crosslink the polymer.


4
Michael W Pantoliano, Robert C Ladner: Computer designed stabilized proteins and method for producing same. Genex Corporation, Saidman Sterne Kessler & Goldstein, March 13, 1990: US04908773 (74 worldwide citation)

Proteins, such as enzymes having enhanced stability are designed through the use of a computer method. The method identifies amino acid residues of a protein which may be replaced with a cysteine residue in order to promote the formation of a protein-stabilizing disulfide bond. The computer-designed ...


5
Michael W Pantoliano, Robert C Ladner: Computer-based method for designing stablized proteins. Genex Corporation, Saidman Sterne Kessler & Goldstein, August 1, 1989: US04853871 (69 worldwide citation)

A computer-based method evaluates the structure of a protein to thereby identify sites in the protein molecule at which the natural amino acid residues can be replaced with cysteine residues in order to permit the formation of a potentially stabilizing disulfide bond.


6
Karl D Hardman: Incremental macromolecule construction methods. Genex Corporation, Saidman Sterne Kessler & Goldstein, July 3, 1990: US04939666 (59 worldwide citation)

A preferred embodiment of the invention is a method for constructing a polypeptide chain having a substantially predetermined conformation. Preferably a known stable well-mapped polypeptide structure is used as a starting point, and additional peptide units are incrementally added on while maintaini ...


7
Philip N Bryan, Michael W Pantoliano: Combining mutations for stabilization of subtilisin. Genex Corporation, Saidman Sterne Kessler & Goldstein, February 5, 1991: US04990452 (33 worldwide citation)

The invention relates to modified subtilisin enzymes which have increased thermal stability. The modified subtilisin enzymes have at least two or more amino acid mutations which confer increased thermal stability. It has been discovered that combining individual stabilizing mutations in subtilisin f ...


8
Kathy J Maugh, David M Anderson, Robert Strausberg, Susan L Strausberg, Russ McCandliss, Tena Wei, David Filpula: Bioadhesive coding sequences. Genex Corporation, Sterne Kessler Goldstein & Fox, September 17, 1991: US05049504 (32 worldwide citation)

Vectors are provided that are capable of expressing, in microbial transformants, a protein having the native amino acid sequence of a bioadhesive precursor protein of a marine animal selected from the group consisting of mussels, barnacles, and oysters. The bioadhesive precursor protein can be expre ...


9
Philip N Bryan, Michele L Rollence, Michael W Pantoliano: Subtilisin with increased thermal stability. Genex Corporation, Saidman Sterne Kessler & Goldstein, December 25, 1990: US04980288 (32 worldwide citation)

Cloned DNA is mutated by creating a single-stranded target region in a cloned DNA segment, and introducing a mutation into the single-stranded target region by treating the target region with a chemical or biological mutagenizing agent capable of introducing mutations into single-stranded DNA. The m ...


10
Wayne E Swann: Immobilization of biological materials in condensed polyalkyleneimine polymers. Genex Corporation, Bernard Rothwell & Brown, February 28, 1984: US04434228 (27 worldwide citation)

Biological materials are immobilized within condensed polyalkyleneimine polymers. Condensation of the polymer is accomplished by bridging the amine groups of polyalkyleneimine polymer chains with a polycarboxylic acid, in the presence of a condensing agent. d