1
Jay M Short: Method of DNA reassembly by interrupting synthesis. Diversa Corporation, Lisa A Haile Ph D, Gray Cary Ware & Freidenrich, October 12, 1999: US05965408 (196 worldwide citation)

Disclosed is a process of performing Sexual PCR which includes generating random polynucleotides by interrupting or blocking a synthesis or amplification process to show or halt synthesis or amplification of at least one polynucleotide, optionally amplifying the polynucleotides, and reannealing the ...


2
Jay M Short: Production of enzymes having desired activities by mutagenesis. Diversa Corporation, Fish & Richardson P C, August 17, 1999: US05939250 (145 worldwide citation)

Disclosed is a process for obtaining an enzyme having a specified enzyme activity derived from a heterogeneous DNA population by screening, for the specified enzyme activity, a library of clones containing DNA from the heterogeneous DNA population which have been exposed to directed mutagenesis towa ...


3
Jay M Short: Directed evolution of thermophilic enzymes. Diversa Corporation, Fish & Richardson P C, November 3, 1998: US05830696 (145 worldwide citation)

Thermostable enzyme are subjected to mutagenesis to produce a thermophilic enzyme which is stable at thermophilic temperature and which has increased activities at least two-fold higher than the activity of the wild-type thermostable enzyme at lower temperatures, which are temperatures of 50.degree. ...


4
Jay M Short: Synthetic ligation reassembly in directed evolution. Diversa Corporation, Jane M Love, Hale and Dorr, March 25, 2003: US06537776 (132 worldwide citation)

Harvesting the full richness of biodiversity is instantly recognized by Diversa Corporation as a powerful means to access both novel molecules having direct commercial utility as well as molecular templates that could be retooled to acquire commercial utility. A directed evolution process for rapid ...


5
Jay M Short: Saturation mutagenesis in directed evolution. Diversa Corporation, Lisa A Haile, Gary Cary Ware & Freidenrich, January 9, 2001: US06171820 (117 worldwide citation)

Disclosed is a rapid and facilitated method of producing from a parental template polynucleotide, a set of mutagenized progeny polynucleotides whereby at each original codon position there is produced at least one substitute codon encoding each of the 20 naturally encoded amino acids. Accordingly, t ...


6
Jay M Short, Tsotne David Djavakhishvili, Gerhard Johann Frey: Exonuclease-mediated nucleic acid reassembly in directed evolution. Diversa Corporation, Lisa A Haile, Greg Shen, Gray Cary Ware & Freidenrich, March 26, 2002: US06361974 (115 worldwide citation)

This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by the use of non-stochastic methods of directed evolution (DirectEvolution™). A particular advantage of exonuclease-mediated reassembly methods is the ability to reassemble nucleic acid strands that would ot ...


7
Jay M Short, Gerhard Johann Frey: End selection in directed evolution. Diversa Corporation, Lisa A Haile, Gray Cary Ware & Freidenrich, May 29, 2001: US06238884 (87 worldwide citation)

A directed evolution process comprising novel methods for generating improved progeny molecules having desirable properties, including, for example, a method for rapid and facilitated production from a parental polynucleotide template, of a set of mutagenized progeny polynucleotides wherein at least ...


8
Jay M Short: Protein activity screening of clones having DNA from uncultivated microorganisms. Diversa Corporation, Fish & Richardson P C, September 28, 1999: US05958672 (84 worldwide citation)

Disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein, e.g. enzyme, activity by screening for a specified protein, e.g. enzyme, activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one un ...


9
Jay M Short: Synthetic ligation reassembly in directed evolution. Diversa Corporation, Jane M Love, Hale and Dorr, August 12, 2003: US06605449 (79 worldwide citation)

Harvesting the full richness of biodiversity is instantly recognized by Diversa Corporation as a powerful means to access both novel molecules having direct commercial utility as well as molecular templates that could be retooled to acquire commercial utility. A directed evolution process for rapid ...


10
Jay M Short, Eric J Mathur: Production and use of normalized DNA libraries. Diversa Corporation, Lisa A Haile, Gary Cary Ware & Friedenrich, December 14, 1999: US06001574 (78 worldwide citation)

Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolate ...