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Church George M, Shendure Jay, Porreca Gregory J, Zhu Jun: Polony fluorescent in situ sequencing beads. President And Fellows Of Harvard College, Church George M, Shendure Jay, Porreca Gregory J, Zhu Jun, IWANICKI John P, September 9, 2005: WO/2005/082098 (73 worldwide citation)

Miniaturized, high-density, bead-based arrays are provided. Methods of producing and using clonal beads and producing and using miniaturized, high density, bead-based arrays are also provided.


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Agresti Jeremy, Chu Liang Yin, Weitz David A, Kim Jin Woong, Rowat Amy, Sommer Morten, Dantas Gautam, Church George: Assays and other reactions involving droplets. President And Fellows Of Harvard College, Agresti Jeremy, Chu Liang Yin, Weitz David A, Kim Jin Woong, Rowat Amy, Sommer Morten, Dantas Gautam, Church George, OYER Timothy J, September 12, 2008: WO/2008/109176 (73 worldwide citation)

The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be perfo ...


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CHURCH George M, VIGNEAULT Francois: ÉTABLISSEMENT À HAUT DÉBIT DUN CODE-BARRES DE CELLULES SIMPLES, HIGH-THROUGHPUT SINGLE CELL BARCODING. PRESIDENT AND FELLOWS OF HARVARD COLLEGE, CHURCH George M, VIGNEAULT Francois, IWANICKI John P, April 12, 2012: WO/2012/048341 (64 worldwide citation)

Methods and compositions for high-throughput, single cell analyses are provided. The methods and compositions can be used for analysis of genomes and transcriptomes, as well as antibody discovery, HLA typing, haplotyping and drug discovery.


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Church George, Porreca Gregory, Shendure Jay: Wobble sequencing. President And Fellows Of Harvard College, Church George, Porreca Gregory, Shendure Jay, IWANCKI John, July 13, 2006: WO/2006/073504 (55 worldwide citation)

Novel methods and compositions for DNA sequencing are provided. The methods described herein are useful for sequencing homopolymeric regions of DNA. The methods also prevent the accumulation of mistakes and inefficiencies in the sequencing reaction.


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Church George M, Porreca Gregory J, Shendure Jay, Rosenbaum Abraham: Nanogrid rolling circle dna sequencing. President And Fellows Of Harvard College, Church George M, Porreca Gregory J, Shendure Jay, Rosenbaum Abraham, IWANICKI John P, October 25, 2007: WO/2007/120208 (26 worldwide citation)

The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sec ...


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Afeyan Noubar, Church George, Jacobson Joseph, Baynes Brian M, Nesmith Kenneth Gabriel, Chapman Brad Alan, Strack Logue Bettina: Methods for assembly of high fidelity synthetic polynucleotides. Codon Devices, Afeyan Noubar, Church George, Jacobson Joseph, Baynes Brian M, Nesmith Kenneth Gabriel, Chapman Brad Alan, Strack Logue Bettina, VINCENT Matthew P, April 27, 2006: WO/2006/044956 (20 worldwide citation)

Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of nucleic acid polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments ...


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Church George, Cerrina Francisco, Baynes Brian M, Pitcher Edmund R: Methods for nucleic acid sorting and synthesis. Codon Devices, Church George, Cerrina Francisco, Baynes Brian M, Pitcher Edmund R, WALLER Patrick R H, November 29, 2007: WO/2007/136736 (17 worldwide citation)

Methods of sorting oligonucleotide sequences are provided. The methods include determining the sequences of oligonucleotides and selectively isolating one or more that are determined to have the desired sequence. Methods of nucleic acid preparation are also disclosed.


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Church George, Jacobson Joseph M, Baynes Brian M: Methods and compositions for aptamer production and uses thereof. Codon Devices, Church George, Jacobson Joseph M, Baynes Brian M, WALLER Patrick R H, November 29, 2007: WO/2007/136833 (13 worldwide citation)

Certain aspects of the present invention provide methods for assembling nucleic acid libraries that represent large numbers of sequence variants. Some embodiments involve generating libraries of RNA molecules that can be subjected to in vivo screens or selections to identify one or more aptamers. Ce ...