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J Wallace Parce, Theo T Nikiforov, Tammy Burd Mehta, Anne R Kopf Sill, Andrea W Chow, Michael R Knapp: Sequencing by incorporation. Caliper Life Sciences, Donald R McKenna, Ann C Petersen, September 12, 2006: US07105300 (159 worldwide citation)

Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carb ...


2
Anne R Kopf Sill, Steven A Sundberg, Andrea W Chow, Claudia L Poglitsch: Dilutions in high throughput systems with a single vacuum source. Caliper Life Sciences, Donald R McKenna, February 22, 2005: US06858185 (129 worldwide citation)

Flow rates in a microfluidic device are modulated after performing serial dilutions by flow reduction channels that draw fluid from the main channel, thus reducing the flow rate. The reduction in flow rate and/or use of smaller dimension channels allow reduced reagent consumption. In addition, multi ...


3
Michael Knapp, John Wallace Parce, Luc J Bousse, Anne R Kopf Sill: Microfluidic sequencing systems. Caliper Life Sciences, Donald R McKenna, Ann C Petersen, July 3, 2007: US07238323 (125 worldwide citation)

Integrated systems, apparatus, software, and methods for performing biochemical analysis, including DNA sequencing, genomic screening, purification of nucleic acids and other biological components and drug screening are provided. Microfluidic devices, systems and methods for using these devices and ...


4
Ring Ling Chien, J Wallace Parce, Andrea W Chow, Anne Kopf Sill: Multi-reservoir pressure control system. Caliper Life Sciences, Donald R McKenna, July 12, 2005: US06915679 (87 worldwide citation)

Improved microfluidic devices, systems, and methods allow selective transportation of fluids within microfluidic channels of a microfluidic network by applying, controlling, and varying pressures at a plurality of reservoirs. Modeling the microfluidic network as a series of nodes connected together ...


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David Chazan: Microfabrication methods and devices. Caliper Life Sciences, Donald R McKenna, June 22, 2004: US06752966 (82 worldwide citation)

Microfabrication methods and devices in which microscale structural elements are provided in an intermediate polymer layer between two planar substrates. Preferred aspects utilize photoimagable or ablatable polymer layers as the intermediate polymer layer.


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Robert S Dubrow, Michael Greenstein, Luc J Bousse, Khushroo Gandhi: Microfluidic sample delivery devices, systems, and methods. Caliper Life Sciences, Ann C Petersen, Donald R McKenna, December 4, 2007: US07303727 (74 worldwide citation)

Methods and apparatus for delivering fluidic materials to sample destinations, including mass spectrometers for analysis are provided. In preferred embodiments, sample aliquots are electrosprayed from tapered spray tips of capillary elements into the orifices of mass spectrometric inlet systems. In ...


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Calvin Y H Chow: Multi-layer microfluidic devices. Caliper Life Sciences, Donald R McKenna, February 22, 2005: US06857449 (62 worldwide citation)

The present invention provides multi-layer microfluidic systems, by providing additional substrate layers, e.g., third, fourth, fifth and more substrate layers, mated with the typically described first and second layers. Microfabricated elements, e.g., grooves, wells and the like, are manufactured i ...


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Ring Ling Chien, J Wallace Parce, Michael Spaid: Mixed mode microfluidic systems. Caliper Life Sciences, Donald R McKenna, Ann C Petersen, January 9, 2007: US07160423 (60 worldwide citation)

Methods and systems that employ hybrid fluid flow profiles for optimized movement of materials through channel networks. These systems employ hybrid pressure-based and electrokinetic based flow systems for moving materials through interconnected channel networks while maintaining interconnection amo ...


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Knapp Michael R, Baker Jill M, Chow Andrea, Kopf Sill Anne, Spaid Michael: Single molecule amplification and detection of dna. Caliper Life Sciences, MCKENNA Donald R, September 30, 2004: WO/2004/083443 (60 worldwide citation)

Methods and systems for performing single molecule amplification for detection, quantification and statistical analysis of rare nucleic acids are provided.