1
Michael C Pirrung, J Leighton Read, Stephen P A Fodor, Lubert Stryer: Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof. Affymax Technologies, Kenneth J Nussbacher, Kevin R Kaster, Vern Norviel, September 1, 1992: US05143854 (1323 worldwide citation)

Polypeptide arrays can be synthesized on a substrate by attaching photoremovable groups to the surface of a substrate, exposing selected regions of the substrate to light to activate those regions, attaching an amino acid monomer with a photoremovable group to the activated regions, and repeating th ...


2
Stephen P A Fodor, Michael C Pirrung, J Leighton Read, Lubert Stryer: Array of oligonucleotides on a solid substrate. Affymax Technologies, Townsend and Townsend Khourie and Crew, August 29, 1995: US05445934 (825 worldwide citation)

A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are exposed to light so as to activate the selected areas. A monomer, also containing a photoremovable group, i ...


3
Stephen P A Fodor, Lubert Stryer, Michael C Pirrung, J Leighton Read: Very large scale immobilized polymer synthesis. Affymax Technologies, Kevin R Kaster, Vern Norviel, June 13, 1995: US05424186 (505 worldwide citation)

A method for synthesizing oligonucleotides on a solid substrate. The method provides for the irradiation of a first predefined region of the substrate without irradiation of a first predefined region of the substrate. The irradiation of a second predefined region of the substrate. The irradiation st ...


4
Willem P C Stemmer, Andreas Crameri: Methods for generating polynucleotides having desired characteristics by iterative selection and recombination. Affymax Technologies, Townsend & Townsend & Crew, September 22, 1998: US05811238 (479 worldwide citation)

A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The p ...


5
Willem P C Stemmer: Methods for in vitro recombination. Affymax Technologies, Townsend & Townsend & Crew, February 25, 1997: US05605793 (472 worldwide citation)

A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present inve ...


6
Willem P C Stemmer, Andreas Crameri: DNA mutagenesis by random fragmentation and reassembly. Affymax Technologies, Townsend & Townsend & Crew, November 3, 1998: US05830721 (465 worldwide citation)

A method for generating libraries of displayed peptides and/or antibodies (Abs) suitable for affinity interaction screening or phenotypic screening comprising: (i) obtaining selected library members comprising a displayed peptide and/or Ab and the corressponding polynucleotide (PN), or copies of it, ...


7
William J Dower, Steven E Cwirla: Recombinant library screening methods. Affymax Technologies, Townsend & Townsend & Crew, December 3, 1996: US05580717 (463 worldwide citation)

Nucleotide sequences encoding proteins of interest are isolated from DNA libraries using bacteriophage to link the protein to the sequence which encodes it. DNA libraries are prepared from cells encoding the protein of interest and inserted into or adjacent to a coat protein of a bacteriophage vecto ...


8
William J Dower, Steven E Cwirla: Recombinant library screening methods. Affymax Technologies, Townsend and Townsend Khourie and Crew, June 27, 1995: US05427908 (435 worldwide citation)

Nucleotide sequences encoding proteins of interest are isolated from DNA libraries using bacteriophage to link the protein to the sequence which encodes it. DNA libraries are prepared from cells encoding the protein of interest and inserted into or adjacent to a coat protein of a bacteriophage vecto ...


9
Stephen P A Fodor, Michael C Pirrung, J Leighton Read, Lubert Stryer: Synthesis and screening of immobilized oligonucleotide arrays. Affymax Technologies, Townsend and Townsend and Crew, April 23, 1996: US05510270 (402 worldwide citation)

A method for synthesizing and screening oligonucleotides on a solid substrate. The method provides for the irradiation of a first predefined region of a substrate comprising immobilized nucleotides on its surface, without irradiation of a second predefined region of the substrate. The irradiation st ...


10
James L Winkler, Stephen P A Fodor, Christopher J Buchko, Debra A Ross, Lois Aldwin: Very large scale immobilized polymer synthesis using mechanically directed flow paths. Affymax Technologies, Kevin R Kaster, Vern Norviel, January 24, 1995: US05384261 (374 worldwide citation)

A method and device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are flowed through the channels, the substrate is rotated by a rotatin ...



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