1
John H Leamon, Kenton L Lohman, Jonathan M Rothberg, Michael P Weiner: Methods of amplifying and sequencing nucleic acids. 454 Life Sciences Corporation, Ivor R Elrifi, Mintz Levin Cohn Ferris Glovsky and Popeo PC, January 29, 2008: US07323305 (335 worldwide citation)

An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the ampl ...


2
Jonathan M Rothberg, Joel S Bader, Scott B Dewell, Keith McDade, John W Simpson, Jan Berka, Christopher M Colangelo: Method of sequencing a nucleic acid. 454 Life Sciences Corporation, Ivor R Elrifi Esq, Mintz Levin Cohn Ferris Glovsky and Popeo PC, July 17, 2007: US07244559 (235 worldwide citation)

Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.


3
Jonathan M Rothberg, Joel S Bader, Scott B Dewell, Keith McDade, John W Simpson, Jan Berka, Christopher M Colangelo: Method of sequencing a nucleic acid. 454 Life Sciences Corporation, Ivor R Elrifi Esq, Mintz Levin Cohn Ferris Glovsky and Popeo PC, September 4, 2007: US07264929 (211 worldwide citation)

Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.


4
Jonathan M Rothberg, Joel S Bader, Scott B Dewell, Keith McDade, John W Simpson, Jan Berka, Christopher M Colangelo: Method of sequencing a nucleic acid. 454 Life Sciences Corporation, Ivor R Elrifi Esq, Mintz Levin Cohn Ferris Glovsky and Popeo PC, May 1, 2007: US07211390 (190 worldwide citation)

Disclosed herein are methods and apparatuses for sequencing a nucleic acid. In one aspect, the method includes annealing a population of circular nucleic acid molecules to a plurality of anchor primers linked to a solid support, and amplifying those members of the population of circular nucleic acid ...


5
Yi Ju Chen, John H Leamon, Kenton L Lohman, Michael T Ronan, Jonathan M Rothberg, Maithreyan Srinivasan, Michael P Weiner: Double ended sequencing. 454 Life Sciences Corporation, Ivor R Elrifi Esq, Mintz Levin Cohn Ferris Glovsky and Popeo PC, July 17, 2007: US07244567 (167 worldwide citation)

This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer ...


6
John H Leamon, Keith E McDade, Joseph M Fierro, James R Knight, Jaran Charumilind, Eugene W Myers Jr, John W Simpson, Greg A Volkmer: Methods of amplifying and sequencing nucleic acids. 454 Life Sciences Corporation, Ivor R Elrifi, Mintz Levin Cohn Ferris Glovsky and Popeo PC, August 18, 2009: US07575865 (163 worldwide citation)

An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the ampl ...


7
Jonathan M Rothberg, Joel S Bader, Scott B Dewell, Keith McDade, John W Simpson, Jan Berka, Christopher M Colangelo, Michael P Weiner: Apparatus and method for sequencing a nucleic acid. 454 Life Sciences Corporation, Ivor R Elrifi Esq, Mintz Levin Cohn Ferris Glovsky and Popeo PC, February 26, 2008: US07335762 (156 worldwide citation)

Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.


8
Jan Berka, Yi Ju Chen, John H Leamon, Steve Lefkowitz, Kenton L Lohman, Vinod B Makhijani, Jonathan M Rothberg, Gary J Sarkis, Maithreyan Srinivasan, Michael P Weiner: Bead emulsion nucleic acid amplification. 454 Life Sciences Corporation, Ivor R Elrifi, Mintz Levin Cohn Ferris Glovsky and Popeo PC, November 30, 2010: US07842457 (124 worldwide citation)

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and appara ...


9
Mcdade Keith E, Fierro Joseph M, Knight James R, Charumilind Jerry, Leamon John H, Myers Eugene W, Simpson John W, Volkmer Greg A: Methods of amplifying and sequencing nucleic acids. 454 Life Sciences Corporation, Mcdade Keith E, Fierro Joseph M, Knight James R, Charumilind Jerry, Leamon John H, Myers Eugene W, Simpson John W, Volkmer Greg A, HOPKINS Brian P, August 2, 2007: WO/2007/086935 (97 worldwide citation)

An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the ampl ...


10
Andrew Griffiths, Dan Tawfik: In vitro sorting method. 454 Life Sciences Corporation, Ivor R Elrifi Esq, Mintz Levin Cohn Ferris Glovsky and Popeo PC, December 29, 2009: US07638276 (71 worldwide citation)

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising of the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within ...