WO/2010/019813 is referenced by 40 patents.
The present invention relates to compositions that facilitate the production of enzymes by genetically modified photosynthetic microorganisms wherein the enzymes contribute to the synthesis of fatty acids, lipids or oils. In some embodiments the compositions are one or more vectors that encode one or more polypeptides that increase production of fatty acids, lipids or oils. The vectors may comprise promoters and other nucleic acid sequences configured for expression of the polypeptides in the chloroplast of non-vascular, photosynthetic organisms. In some embodiments the compositions are genetically altered photosynthetic microorgansims or genetically altered chloroplasts. The present invention provides methods of producing one or more proteins, including fatty acid synthesizing enzymes in a photosynthetic microorganism. In some embodiments the present invention is a method of preparing a fuel. The methods disclosed herein allow for the production of crude or refined biofuels, including ethanol.