WO/2008/109176 is referenced by 73 patents.

The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.

Title
Assays and other reactions involving droplets
Application Number
PCT/US2008/003185
Publication Number
2008/109176
Application Date
March 7, 2008
Publication Date
September 12, 2008
Inventor
Church George
Dantas Gautam
Sommer Morten
Rowat Amy
Kim Jin Woong
Weitz David A
Chu Liang Yin
Agresti Jeremy
Agent
OYER Timothy J
Assignee
Church George
Dantas Gautam
Sommer Morten
Rowat Amy
Kim Jin Woong
Weitz David A
Chu Liang Yin
Agresti Jeremy
President And Fellows Of Harvard College
IPC
B01J 13/00
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