WO/2006/102233 is referenced by 15 patents.

A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, all cells in a biological sample are fluorescently labelled, but only the target cells are also magnetically labelled. A small, permanent magnet is inserted directly into the chamber containing the labelled sample. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The cells are illuminated and the images of the fluorescent light emitted by the target cells are captured by a CCD camera. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample.

Method and apparatus for imaging target components in a biological sample using permanent magnets
Application Number
Publication Number
Application Date
March 20, 2006
Publication Date
September 28, 2006
Terstappen Leon W M M
Tibbe Arjan
ACETO Joseph
Terstappen Leon W M M
Tibbe Arjan
Immunivest Corporation
G01N 33/53
C02F 01/48
G01N 33/553
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