The present invention relates to a process for preparing anthrax protective antigen protein from E.coli using fed batch culture. This process creates a constitutively expressing system for rapid, efficient, cost-effective and high-level production of anthrax PA from E.coli. The steps of the process involves, transforming E.coli DH5$g(a) cells with the recombinant constitutive expression plasmid containing the PA gene to obtain recombinant DH5$g(a) cells and testing the PA expression by lysis of said cells followed by denaturing gel electrophoresis and Western Blotting technique using PA antibodies. This is followed by fermentation and harvesting of the high cell density cells. The said cells are solubilized using 6-8 Molar Urea and separated by centrifugation. The urea denatured PA is isolated from said supernatant and purified and thereafter eluted.

Title
High level constitutive production of anthrax protective antigen
Application Number
PCT/IN2001/000215
Publication Number
2003/040179
Application Date
December 7, 2001
Publication Date
May 15, 2003
Inventor
Chauhan Vibha
Waheed Syed Mohsin
Bhatnagar Rakesh
Agent
GUPTA Bhartee
Assignee
Chauhan Vibha
Waheed Syed Mohsin
Bhatnagar Rakesh
IPC
C07K 14/32
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