Methods and apparatus for detecting polynucleotide hybridization in luminescence-based assays. The methods may include (1) allele-specific hybridization, using luminescence detection, (2) allele-specific oligonucleotide ligation, using dye-labeled oligonucleotide ligation with luminescence resonance energy transfer (FRET) detection, and (3) allele-specific nucleotide incorporation, using primer extension with luminescence polarization (FP) detection. More specifically, the methods may include (1) locating a sample containing a nucleic acid material at an examination site, (2) illuminating the sample, (3) detecting light transmitted from the sample, and (4) determining the presence, absence, and/or identity of a nucleic acid target in the sample using the light transmitted from the sample.

Title
Methods and apparatus for detecting nucleic acid polymorphisms
Application Number
PCT/US2000/006841
Publication Number
2000/055372
Application Date
March 15, 2000
Publication Date
September 21, 2000
Inventor
Panfili Peter R
Zhang Klompus Yan
Razvi Enal S
Leytes Lev J
Richey James S
Owicki John C
Modlin Douglas N
French Todd E
Agent
ABNEY James R
Assignee
Panfili Peter R
Zhang Klompus Yan
Razvi Enal S
Leytes Lev J
Richey James S
Owicki John C
Modlin Douglas N
French Todd E
Ljl Biosystems
IPC
C12Q 01/68
C12Q 01/48
C07K 07/08
C07K 07/06
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