This invention provides compositions for use in implanting cells into an animal comprising cells (which may be dissociated cells and/or cell aggregates); a biodegradable, biocompatible polymer which forms a hydrogel upon cross-linking by multivalent ions; a soluble salt of a multivalent ion; and a sparingly soluble salt of a multivalent ion, these components being combined into a mixture which forms a partially hardened, injectable hydrogel in which the cells are uniformly suspended, the consistency of the mixture being suitable for implanting the partially hardened hydrogel mixture into the animal, where the implanted, partially hardened hydrogel forms in situ a fully hardened hydrogel containing the cells. Preferably, the mixture also contains a biocompatible sequestrant which competes with the biocompatible polymer for binding of the multivalent cross-linking ion. The invention also provides methods for implanting cells in an animal using the composition.

Title
Improved hydrogel composition
Application Number
PCT/US1997/022860
Publication Number
1998/025575
Application Date
December 10, 1997
Publication Date
June 18, 1998
Inventor
Atala Anthony
Nelson Gordon P
Zhou Tao
Borland Kermit M
Agent
POSORSKE Laurence H
Assignee
Children S Medical Center Corporation
Reprogenesis
IPC
A61K 47/30
A61F 02/02
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