3907645 is referenced by 18 patents and cites 5 patents.

Enzyme preparations which will convert cholesterol to .DELTA..sup.4 -cholestenone and hydrogen peroxide are obtained from certain Nocardia species belonging to the Mycobacterium rhodocrous group. The preparations have a cholesterol oxidase specific activity of at least 1 unit per 5 mg of protein nitrogen and, when in liquid form, a potency of at least 10.sup..sup.-2 units/ml. They are prepared by growing the organism and recovering the enzyme preparation, preferably by extracting the harvested cells with a surface active agent such as Triton X-100. The enzyme preparations are used to assay for cholesterol by measuring the amount in which one of the products of the cholesterol oxidase reaction, preferably hydrogen peroxide, is formed or the quantity in which oxygen is used.

Title
Cholesterol assay
Application Number
05/435,892
Publication Number
3907645
Application Date
January 23, 1974
Publication Date
September 23, 1975
Inventor
Richmond William
Assignee
National Research Development Corporation
IPC
G01n 31/14
C12N 09/04
C12Q 01/26
C12Q 01/60
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