09957499 cites 666 patents.

The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of subjecting an impure preparation comprising in vitro synthesized mRNA to a denaturing condition, and purifying the mRNA from the impure preparation from step (a) by tangential flow filtration, wherein the mRNA purified from step (b) is substantially free of prematurely aborted RNA sequences and/or enzyme reagents used in in vitro synthesis.

Title
Methods for purification of messenger RNA
Application Number
14/775915
Publication Number
9957499 (B2)
Application Date
March 14, 2014
Publication Date
May 1, 2018
Inventor
Shirang Karve
Cambridge
MA, US
Anusha Dias
Cambridge
MA, US
Frank DeRosa
Cambridge
MA, US
Michael Heartlein
Cambridge
MA, US
Agent
Proskauer Rose
Fangh Chen
Assignee
TRANSLATE BIO
MA, US
IPC
C07H 21/02
C12N 15/10
C07H 21/00
G01N 1/34
View Original Source