09850269 cites 666 patents.

The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of (a) precipitating mRNA from an impure preparation; (b) subjecting the impure preparation comprising precipitated mRNA to a purification process involving membrane filtration such that the precipitated mRNA is captured by a membrane; and (c) eluting the captured precipitated mRNA from the membrane by re-solubilizing the mRNA, thereby resulting in a purified mRNA solution. In some embodiments, a purification process involving membrane filtration suitable for the present invention is tangential flow filtration.

Title
Methods for purification of messenger RNA
Application Number
14/696140
Publication Number
9850269 (B2)
Application Date
April 24, 2015
Publication Date
December 26, 2017
Inventor
Michael Heartlein
Boxborough
MA, US
Shrirang Karve
Lexington
MA, US
Anusha Dias
Lexington
MA, US
Frank DeRosa
Chelmsford
MA, US
Agent
Proskauer Rose
Fangli Chen
Assignee
Translate Bio
MA, US
IPC
C07H 21/02
C12N 15/10
C07H 1/06
C12Q 1/68
C07H 21/00
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