09648856 cites 123 patents.

Genetically modified non-human animals are provided that express an immunoglobulin variable domain that comprises at least one histidine, wherein the at least one histidine is encoded by a substitution of a non-histidine codon in the germline of the animal with a histidine codon, or the insertion of a histidine codon in a germline immunoglobulin nucleic acid sequence. Immunoglobulin genes comprising histidines in one or more CDRs, in an N-terminal region, and/or in a loop 4 region are also provided. Immunoglobulin variable domains comprising one or more histidines (e.g., histidine clusters) substituted for non-antigen-binding non-histidine residues. Non-human animals that are progeny of animals comprising modified heavy chain variable loci (V, D, J segments), modified light chain variable loci (V, J segments), and rearranged germline light chain genes (VJ sequences) are also provided. Non-human animals that make immunoglobulin domains that bind antigens in a pH-sensitive manner are provided.

Title
Non-human animals expressing pH-sensitive immunoglobulin sequences
Application Number
14/85424
Publication Number
9648856 (B2)
Application Date
November 20, 2013
Publication Date
May 16, 2017
Inventor
Andrew J Murphy
Croton-on-Hudson
NY, US
Joel H Martin
Putnam Valley
NY, US
Lynn MacDonald
White Plains
NY, US
John McWhirter
Tarrytown
NY, US
Agent
Ilona Gont
Rita S Wu
Brownstein Hyatt Farber Schreck
Assignee
Regeneron Pharmaceuticals
NY, US
IPC
C12N 15/85
C07K 16/00
A01K 67/027
C12P 21/00
C12N 15/00
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