09593157 cites 53 patents.

Described are polypeptides and their use for screening and drug discovery. More specifically, the disclosure provides chimeric polypeptides comprising a membrane protein, in particular a GPCR, fused to a binding domain, wherein the binding domain is directed against and/or specifically binds to the membrane protein. In particular, the chimeric polypeptides are single proteins wherein, in an intramolecular reaction, the binding domain stabilizes the membrane protein in a conformation of interest. Also provided are nucleic acid sequences encoding such chimeric polypeptides, cells capable of expressing such chimeric polypeptides as well as cellular compositions derived thereof. Also screening methods for compounds using the chimeric polypeptides.

Title
Chimeric polypeptides comprising G protein-coupled receptors and VHH antibodies
Application Number
14/764548
Publication Number
9593157 (B2)
Application Date
January 30, 2014
Publication Date
March 14, 2017
Inventor
Els Pardon
Lubbeek
BE
Toon Laeremans
Dworp
BE
Jan Steyaert
Beersel
BE
Agent
TraskBritt P C
Assignee
Vrije Universiteit Brussel
BE
VIB VZW
BE
IPC
C07K 16/26
C07K 16/18
C07K 16/00
G01N 33/566
C12N 15/62
C07K 16/28
C07K 14/705
C07K 14/72
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