09156778 cites 7 patents.

Disclosed is a method for lowering the detection limit in a method of detecting a nucleic acid comprising (i) contacting a solution comprising a first PNA with a substrate having a second PNA affixed thereto, the second PNA comprising at least one trans-cyclopentane residue, wherein the first PNA has two linker-attached biotins attached thereto and the first and second PNAs being complementary to different portions of a target DNA; (ii) contacting a sample suspected of containing the nucleic acid with the first and second PNAs; and (iii) determining the presence of the reporter molecule on the substrate. Also disclosed are a detection assay and a kit for detecting a target nucleic acid.

Title
Cross-coupled peptide nucleic acids for detection of nucleic acids of pathogens
Application Number
13/592490
Publication Number
9156778 (B2)
Application Date
August 23, 2012
Publication Date
October 13, 2015
Inventor
Christopher Micklitsch
Rockville
MD, US
Daniel H Appella
Rockville
MD, US
Agent
Leydig Voit & Mayer
Assignee
The United States of America represented by the Secretary Department of Health and Human Services
DC, US
IPC
C07K 14/00
C07C 303/40
C07C 271/24
C12Q 1/70
View Original Source