08329422 is referenced by 14 patents and cites 52 patents.

The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry and the CELLSPOTTERĀ® fluorescent microscopy imaging system. Analyzing damaged cells has shown to be important. However, there are two sources of damage: in vivo and in vitro. Damage in vivo occurs by apoptosis, necrosis, or immune response. Damage in vitro occurs during sample acquisition, handling, transport, processing, or analysis. It is therefore desirable to confine, reduce, eliminate, or at least qualify in vitro damage to prevent it from interfering in analysis. Described herein are methods to diagnose, monitor, and screen disease based on circulating rare cells, including malignancy as determined by CTC, clusters, fragments, and debris. Also provided are kits for assaying biological specimens using these methods.

Title
Analysis of circulating tumor cells, fragments, and debris
Application Number
12/917630
Publication Number
8329422 (B2)
Application Date
November 2, 2010
Publication Date
December 11, 2012
Inventor
Steven Gross
Ambler
PA, US
Shawn Mark O Hara
Richboro
PA, US
Leon W M M Terstappen
Amsterdam
NL
Herman Rutner
Hatboro
PA, US
Madeline Repollet
Fort Washington
PA, US
Christopher Larson
Springfield
PA, US
Galla Chandra Rao
Princeton Jct.
NJ, US
Agent
Ruby T Hope
Assignee
Veridex
NJ, US
IPC
G01N 1/18
G01N 33/553
G01N 33/574
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