08304230 is referenced by 32 patents and cites 598 patents.

The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).

Title
Microfluidic device for cell separation and uses thereof
Application Number
11/726230
Publication Number
8304230 (B2)
Application Date
March 21, 2007
Publication Date
November 6, 2012
Inventor
Ravi Kapur
Sharon
MA, US
George Truskey
Durham
NC, US
Mehmet Toner
Wellesley
MA, US
Agent
Fish & Richardson P C
Assignee
The General Hospital Corporation
MA, US
IPC
C12M 3/00
C12M 1/34
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