08110101 is referenced by 15 patents and cites 20 patents.

A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. A small, permanent magnet is inserted directly into the chamber containing the labeled sample. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The cells are illuminated and the images of the fluorescent light emitted by the target cells are captured by a CCD camera. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample.

Title
Method and apparatus for imaging target components in a biological sample using permanent magnets
Application Number
11/897471
Publication Number
8110101 (B2)
Application Date
August 30, 2007
Publication Date
February 7, 2012
Inventor
Leon W M M Terstappen
Huntingdon Valley
PA, US
Arjan G J Tibbe
Deventer
NL
Agent
Ruby T Hope
Assignee
Veridex
NJ, US
IPC
G01N 33/48
B03C 1/30
B03C 1/02
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