07828968 is referenced by 18 patents and cites 15 patents.

A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, fluorescently labeled target cells are linked to magnetic particles or beads. In one embodiment, a small, permanent magnet is inserted directly into the chamber containing the labeled cells. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The magnet is removed from the sample and illuminated with fluorescent light emitted by the target cells captured by a CCD camera. In another embodiment, a floater having a permanent magnet allows the target cells to line up along a single imaging plane within the sample solution. Image analysis can be performed with a novel algorithm to provide a count of the cells on the surface, reflecting the target cell concentration of the original sample.

Title
Method and apparatus for imaging target components in a biological sample using permanent magnets
Application Number
12/31807
Publication Number
7828968 (B2)
Application Date
February 15, 2008
Publication Date
November 9, 2010
Inventor
Leon W M M Terstappen
Huntingdon Valley
PA, US
Arjan G J Tibbe
Deventer
NL
Assignee
Veridex
NJ, US
IPC
B03C 1/30
B03C 1/02
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