07820799 is referenced by 17 patents and cites 108 patents.

The present application provides methods of purifying polypeptides having a Fc region, for example, antibodies or antibody fusions, by adsorbing the polypeptides to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed polypeptides. The present application also features methods of eluting the purified polypeptide as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.

Title
Methods of purifying Fc region containing proteins
Application Number
11/455203
Publication Number
7820799 (B2)
Application Date
June 16, 2006
Publication Date
October 26, 2010
Inventor
Timothy Iskra
Derry
NH, US
Ranganathan Godavarti
Burlington
MA, US
Agent
Alston & Bird
Assignee
Wyeth
NJ, US
Janssen Alzheimer Immunotherapy
IE
IPC
C07K 1/22
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