07645596 is referenced by 87 patents and cites 612 patents.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA poly-merase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

Title
Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
Application Number
10/709436
Publication Number
7645596 (B2)
Application Date
May 5, 2004
Publication Date
January 12, 2010
Inventor
Mark A Hayes
Gilbert
AZ, US
Ian Gould
Phoenix
AZ, US
Daniel J B Williams
Tempe
AZ, US
Thomas J Taylor
Tempe
AZ, US
Peter Williams
Phoenix
AZ, US
Agent
Brown Rudnick
Adam Schoen
Thomas Meyers
Assignee
Arizona Board of Regents
AZ, US
IPC
C07H 21/04
C07H 21/02
C12Q 1/68
C12P 19/34
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