07566538 is referenced by 8 patents and cites 93 patents.

Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.

Title
Sequencing by incorporation
Application Number
12/17859
Publication Number
7566538 (B2)
Application Date
January 22, 2008
Publication Date
July 28, 2009
Inventor
Michael R Knapp
Redwood City
CA, US
Andrea W Chow
Los Altos
CA, US
Anne R Kopf Sill
Portola Valley
CA, US
Tammy Burd Mehta
San Jose
CA, US
Theo T Nikiforov
San Jose
CA, US
J Wallace Parce
Palo Alto
CA, US
Agent
Cardinal Law Group
Assignee
Caliper Lifesciences
CA, US
IPC
C07H 21/04
C07H 21/02
C07H 21/00
C12P 19/34
C12Q 1/68
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