Various forms of surgical instruments are disclosed. In various embodiments, the instrument includes a cartridge supporting assembly for operably supporting a staple cartridge therein. The cartridge supporting assembly may be responsive to firing and retraction motions applied thereto from a firing assembly. An anvil may be operably coupled to an anvil closure assembly. The anvil closure assembly may be constructed to selectively move the anvil in a proximal direction toward the cartridge supporting assembly to enable a portion of tissue to be clamped between a cartridge supported by the cartridge supporting assembly and the anvil under a predetermined amount of compression. The device may further include a compression limiting assembly that interacts with the anvil closure assembly to prevent further travel of the anvil in the proximal direction toward the cartridge supporting assembly when the predetermined amount of compression has been attained.