07294503 is referenced by 214 patents and cites 122 patents.

A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix. Biological material within the droplets can be analyzed and/or sorted by detecting a predetermined characteristic of the biological sample in each droplet and sorting the droplet accordingly.

Title
Microfabricated crossflow devices and methods
Application Number
9/953103
Publication Number
7294503 (B2)
Application Date
September 14, 2001
Publication Date
November 13, 2007
Inventor
Todd Thorsen
Pasadena
CA, US
Stephen R Quake
Pasadena
CA, US
Agent
Townsend and Townsend and Crew
Ted Apple
Steven W Collier
Assignee
California Institute of Technology
CA, US
IPC
G01N 33/50
C12M 1/34
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