07105300 is referenced by 162 patents and cites 94 patents.

Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.

Title
Sequencing by incorporation
Application Number
10/413049
Publication Number
7105300 (B2)
Application Date
April 14, 2003
Publication Date
September 12, 2006
Inventor
Michael R Knapp
Redwood City
CA, US
Andrea W Chow
Los Altos
CA, US
Anne R Kopf Sill
Portola Valley
CA, US
Tammy Burd Mehta
San Jose
CA, US
Theo T Nikiforov
San Jose
CA, US
J Wallace Parce
Palo Alto
CA, US
Agent
Ann C Petersen
Donald R McKenna
Assignee
Caliper Life Sciences
CA, US
IPC
C07H 19/04
C12P 19/34
C12Q 1/68
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