06960437 is referenced by 334 patents and cites 145 patents.

The present invention provides microfluidic devices and methods using the same in various types of thermal cycling reactions. Certaom devices include a rotary microfluidic channel and a plurality of temperature regions at different locations along the rotary microfluidic channel at which temperature is regulated. Solution can be repeatedly passed through the temperature regions such that the solution is exposed to different temperatures. Other microfluidic devices include an array of reaction chambers formed by intersecting vertical and horizontal flow channels, with the ability to regulate temperature at the reaction chambers. The microfluidic devices can be used to conduct a number of different analyses, including various primer extension reactions and nucleic acid amplification reactions.

Title
Nucleic acid amplification utilizing microfluidic devices
Application Number
10/118466
Publication Number
6960437 (B2)
Application Date
April 5, 2002
Publication Date
November 1, 2005
Inventor
Stephen R Quake
San Marino
CA, US
Jian Liu
Pasadena
CA, US
Markus M Enzelberger
Esslingen
DE
Agent
Townsend and Townsend and Crew
Assignee
California Institute of Technology
CA, US
IPC
C12M 003/00
C12P 019/34
C12Q 001/68
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