06858185 is referenced by 135 patents and cites 93 patents.

Flow rates in a microfluidic device are modulated after performing serial dilutions by flow reduction channels that draw fluid from the main channel, thus reducing the flow rate. The reduction in flow rate and/or use of smaller dimension channels allow reduced reagent consumption. In addition, multiple flow reduction channels are used for multiple concentration measurements and for performing multiple assays simultaneously on a single sample. Also included are microfluidic devices and integrated systems for performing assays using serial dilutions, single pressure sources, multiple concentration measurements, and reduced reagent consumption. Devices comprising flow reduction channels are also used to suppress pressure perturbations from spontaneous injection.

Title
Dilutions in high throughput systems with a single vacuum source
Application Number
9/645104
Publication Number
6858185 (B1)
Application Date
August 23, 2000
Publication Date
February 22, 2005
Inventor
Claudia L Poglitsch
Sunnyvale
CA, US
Andrea W Chow
Los Altos
CA, US
Steven A Sundberg
San Francisco
CA, US
Anne R Kopf Sill
Portola Valley
CA, US
Agent
Donald R McKenna
Assignee
Caliper Life Sciences
CA, US
IPC
B01L 003/02
G01N 033/00
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