06852487 is referenced by 181 patents and cites 56 patents.

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.

Title
Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
Application Number
8/794851
Publication Number
6852487 (B1)
Application Date
February 4, 1997
Publication Date
February 8, 2005
Inventor
Monib Zirvi
New York
NY, US
Herman Blok
Wemeldinge
NL
Maria Kempe
Minneapolis
MN, US
Robert P Hammer
Baton Rouge
LA, US
George Barany
Falcon Heights
MN, US
Francis Barany
New York
NY, US
Agent
Nixon Peabody
Assignee
Regents of the University of Minnesota
MN, US
Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
LA, US
Cornell Research Foundation
NY, US
IPC
C07H 021/04
C07H 021/02
G01N 033/543
C12Q 001/68
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