06825463 is referenced by 10 patents and cites 2 patents.

A universal interface for continuous on-line liquid sample introduction directly to the time-of-flight mass spectrometer, which can further promote throughput and utility of MALDI-TOF MS, is disclosed. Preferably, the liquid sample includes a matrix, either solid or liquid, for use in matrix-assisted-laser-desorption-ionization, most particularly in a time-of-flight mass spectrometer which can further promote throughput and utility of MALDI-TOF MS. In the method of the invention, the same samples and matrices, both solid and liquid, can be used as in conventional MALDI. In practice of the method of the invention, a solution of sample containing, e.g., peptide and matrix is infused directly into the source chamber of a mass spectrometer at subatmospheric pressure, deposited on a moving sample holder, such as a rotating quartz wheel, and desorbed by, e.g., a nitrogen laser. The method of the invention is particularly amenable to multiplexing, the parallel deposition of multiple samples, e.g., from a capillary array or microchip channels, with subsequent sequential desorption with a scanning laser. This format is particularly useful for high throughput MS analysis. Also disclosed is an off-line deposition chamber and a general method of preparing a sample for analysis that results in the homogeneous deposition of small quantities of sample at improved reproducibility on any appropriately configured sample receptor. This format of sample preparation is particularly useful with existing commercial mass spectrometers.

Title
On-line and off-line deposition of liquid samples for matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectroscopy
Application Number
10/132064
Publication Number
6825463 (B2)
Application Date
April 25, 2002
Publication Date
November 30, 2004
Inventor
Jan Preisler
Lelekovice
US
Frantisek Foret
Malden
MA, US
Barry L Karger
Newton
MA, US
Agent
Weingarten Schurgin Gagnebin & Lebovici
US
Assignee
Northeastern University
MA, US
IPC
H01J 49/00
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