06605459 is referenced by 4 patents and cites 1 patents.

Assays, preferably high throughput assays, for determining cysteine concentration, cysteine synthase activity, and identifying herbicides, fungicides, bactericides, and insecticides. Cysteine concentration is quantitated by contacting cysteine with a coumarin dye capable of conjugating with cysteine but not to O-acetyl serine or sulfide; exciting the conjugate with UV light; and detecting fluorescent light emitted by the conjugate. Cysteine synthase activity is determined by combining O-acetyl-L-serine, sulfide and cysteine synthase to form a reaction mixture under conditions suitable for cysteine production; contacting the reaction mixture with an appropriate coumarin dye; subjecting the reaction mixture to UV light; and detecting fluorescent light emission.

Title
Methods for measuring cysteine and determining cysteine synthase activity
Application Number
9/905290
Publication Number
6605459 (B2)
Application Date
July 13, 2001
Publication Date
August 12, 2003
Inventor
Gordon Nye
Cary
NC, US
John Crawford
Raleigh
NC, US
Beth Lanning
Cary
NC, US
John Rice
Pittsboro
NC, US
Agent
Deborah H Spencer
US
Timothy G Hofmeyer
US
Laura L Kiefer
US
Assignee
Paradigm Genetics
NC, US
IPC
C12N 9/88
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