06577754 is referenced by 10 patents.

A physics based model of the absorption of light by histological stains used to measure the amount of one or more stains at locations within tissue is disclosed. The subsequent analysis results in several improvements in the detection of tissue on a slide, improvements to autofocus algorithms so focusing during image acquisition is confined to tissue, improvements to image segmentation and identification of tissued and its features, improvements to the identification of stain where multiple stains are used, and improvements to the quantification of the extent of staining. The invention relates to the application of these improvements to stain detection and quantification to provide for objective comparison between tissues and closer correlation between the presentations of such features and concurrent patterns of gene or protein expression.

Title
Robust stain detection and quantification for histological specimens based on a physical model for stain absorption
Application Number
10/158486
Publication Number
6577754 (B2)
Application Date
May 29, 2002
Publication Date
June 10, 2003
Inventor
Michael Fuhrman
Pittsburgh
PA, US
Othman Abdulkarim
Pittsburgh
PA, US
Ronald Stone
Pittsburgh
PA, US
Agent
Nanda P B A Kumar
US
Lawrence B Ebert
US
Louis M Heidelberger
US
Assignee
TissueInformatics
PA, US
IPC
G06K 9/00
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